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MMS19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MMS19 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110374-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MMS19 (NM_022362), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MET18 | MMS19-like protein | MET18 homolog | MMS19L | HMMS19 | Homolog of yeast MMS19 | MMS19
Origin Species
Human
NCBI GenBank
NM_022362
SwissProt
Q96T76

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGEPVSSHR PYPLPRSLVR VMAAAAAVEA AAPMGALWGL VHDFVVGQQE GPADQVAADV
61KSGNYTVLQV VEALGSSLEN PEPRTRARAI QLLSQVLLHC HTLLLEKEVV HLILFYENRL
121KDHHLVIPSV LQGLKALSLC VALPPGLAVS VLKAIFQEVH VQSLPQVDRH TVYNIITNFM
181RTREEELKSL GADFTFGFIQ VMDGEKDPRN LLVAFRIVHD LISRDYSLGP FVEELFEVTS
241CYFPIDFTPP PNDPHGIQRE DLILSLRAVL ASTPRFAEFL LPLLIEKVDS EVLSAKLDSL
301QTLNACCAVY GQKELKDFLP SLWASIRREV FQTASERVEA EGLAALHSLT ACLSRSVLRA
361DAEDLLDSFL SNILQDCRHH LCEPDMKLVW PSAKLLQAAA GASARACDSV TSNVLPLLLE
421QFHKHSQSSQ RRTILEMLLG FLKLQQKWSY EDKDQRPLNG FKDQLCSLVF MALTDPSTQL
481QLVGIRTLTV LGAQPDLLSY EDLELAVGHL YRLSFLKEDS QSCRVAALEA SGTLAALYPV
541AFSSHLVPKL AEELRVGESN LTNGDEPTQC SRHLCCLQAL SAVSTHPSIV KETLPLLLQH
601LWQVNRGNMV AQSSDVIAVC QSLRQMAEKC QQDPESCWYF HQTAIPCLLA LAVQASMPEK
661EPSVLRKVLL EDEVLAAMVS VIGTATTHLS PELAAQSVTH IVPLFLDGNV SFLPENSFPS
721RFQPFQDGSS GQRRLIALLM AFVCSLPRNV EIPQLNQLMR ELLELSCCHS CPFSSTAAAK
781CFAGLLNKHP AGQQLDEFLQ LAVDKVEAGL GSGPCRSQAF TLLLWVTKAL VLRYHPLSSC
841LTARLMGLLS DPELGPAAAD GFSLLMSDCT DVLTRAGHAE VRIMFRQRFF TDNVPALVQG
901FHAAPQDVKP NYLKGLSHVL NRLPKPVLLP ELPTLLSLLL EALSCPDCVV QLSTLSCLQP
961LLLEAPQVMS LHVDTLVTKF LNLSSSPSMA VRIAALQCMH ALTRLPTPVL LPYKPQVIRA
1021LAKPLDDKKR LVRKEAVSAR GEWFLLGSPG S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1051 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.