Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MMP25 / Leukolysin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110350-100
Catalog Number: LS-G110350
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MMP25 (NM_022468), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Matrix metalloproteinase 25 | MMP20A | MMPL1 | MT6MMP | Matrix metallopeptidase 25 | Matrix metallopeptidase-like 1 | MMP-25 | MT6-MMP | MTMMP6 | Leukolysin | Matrix metalloproteinase 20 | Matrix metalloproteinase-25 | MMP25 | MT-MMP 6 | MT-MMP6
- Origin Species
- Human
- NCBI GenBank
- NM_022468
- SwissProt
- Q9NPA2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRLRLRLLAL LLLLLAPPAR APKPSAQDVS LGVDWLTRYG YLPPPHPAQA QLQSPEKLRD61AIKVMQRFAG LPETGRMDPG TVATMRKPRC SLPDVLGVAG LVRRRRRYAL SGSVWKKRTL121TWRVRSFPQS SQLSQETVRV LMSYALMAWG MESGLTFHEV DSPQGQEPDI LIDFARAFHQ181DSYPFDGLGG TLAHAFFPGE HPISGDTHFD DEETWTFGSK DGEGTDLFAV AVHEFGHALG241LGHSSAPNSI MRPFYQGPVG DPDKYRLSQD DRDGLQQLYG KAPQTPYDKP TRKPLAPPPQ301PPASPTHSPS FPIPDRCEGN FDAIANIRGE TFFFKGPWFW RLQPSGQLVS PRPARLHRFW361EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG GARPLTELGL PPGEEVDAVF421SWPQNGKTYL VRGRQYWRYD EAAARPDPGY PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG481AHYWRFPKNS IKTEPDAPQP MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQAA541GRWPAPIPLL LLPLLVGGVA SRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
562 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.