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MMP15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MMP15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MMP15 antibodies →
$504.00each
Catalog Number: LS-G99754-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MMP15 (NM_002428), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MT2-MMP | MT2MMP | MTMMP2 | MMP-15 | MT-MMP 2 | Matrix metalloproteinase-15 | MMP15 | SMCP-2
NCBI GenBank
NM_002428
SwissProt
P51511

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.81 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSDPSAPGR PGWTGSLLGD REEAARPRLL PLLLVLLGCL GLGVAAEDAE VHAENWLRLY
61GYLPQPSRHM STMRSAQILA SALAEMQRFY GIPVTGVLDE ETKEWMKRPR CGVPDQFGVR
121VKANLRRRRK RYALTGRKWN NHHLTFSIQN YTEKLGWYHS MEAVRRAFRV WEQATPLVFQ
181EVPYEDIRLR RQKEADIMVL FASGFHGDSS PFDGTGGFLA HAYFPGPGLG GDTHFDADEP
241WTFSSTDLHG NNLFLVAVHE LGHALGLEHS SNPNAIMAPF YQWKDVDNFK LPEDDLRGIQ
301QLYGTPDGQP QPTQPLPTVT PRRPGRPDHR PPRPPQPPPP GGKPERPPKP GPPVQPRATE
361RPDQYGPNIC DGDFDTVAML RGEMFVFKGR WFWRVRHNRV LDNYPMPIGH FWRGLPGDIS
421AAYERQDGRF VFFKGDRYWL FREANLEPGY PQPLTSYGLG IPYDRIDTAI WWEPTGHTFF
481FQEDRYWRFN EETQRGDPGY PKPISVWQGI PASPKGAFLS NDAAYTYFYK GTKYWKFDNE
541RLRMEPGYPK SILRDFMGCQ EHVEPGPRWP DVARPPFNPH GGAEPGADSA EGDVGDGDGD
601FGAGVNKDGG SRVVVQMEEV ARTVNVVMVL VPLLLLLCVL GLTYALVQMQ RKGAPRVLLY
661CKRSLQEWV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

669 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.