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MLLT10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MLLT10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MLLT10 antibodies →
$725.00each
Catalog Number: LS-G116512-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AF10(MLLT10) (NM_004641), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AF10 | Protein AF-10 | MLLT10 | Type I AF10 protein | Type III AF10 protein | Translocated to | 10 | Type IV AF10 protein
Origin Species
Human
NCBI GenBank
NM_004641
SwissProt
P55197

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVSSDRPVSL EDEVSHSMKE MIGGCCVCSD ERGWAENPLV YCDGHGCSVA VHQACYGIVQ
61VPTGPWFCRK CESQERAARV RCELCPHKDG ALKRTDNGGW AHVVCALYIP EVQFANVSTM
121EPIVLQSVPH DRYNKTCYIC DEQGRESKAA TGACMTCNKH GCRQAFHVTC AQFAGLLCEE
181EGNGADNVQY CGYCKYHFSK LKKSKRGSNR SYDQSLSDSS SHSQDKHHEK EKKKYKEKDK
241HKQKHKKQPE PSPALVPSLT VTTEKTYTST SNNSISGSLK RLEDTTARFT NANFQEVSAH
301TSSGKDVSET RGSEGKGKKS SAHSSGQRGR KPGGGRNPGT TVSAASPFPQ GSFSGTPGSV
361KSSSGSSVQS PQDFLSFTDS DLRNDSYSHS QQSSATKDVH KGESGSQEGG VNSFSTLIGL
421PSTSAVTSQP KSFENSPGDL GNSSLPTAGY KRAQTSGIEE ETVKEKKRKG NKQSKHGPGR
481PKGNKNQENV SHLSVSSASP TSSVASAAGS ITSSSLQKSP TLLRNGSLQS LSVGSSPVGS
541EISMQYRHDG ACPTTTFSEL LNAIHNDRGD SSTLTKQELK FIGIYNSNDV AVSFPNVVSG
601SGSSTPVSSS HLPQQSSGHL QQVGALSPSA VSSAAPAVAT TQANTLSGSS LSQAPSHMYG
661NRSNSSMAAL IAQSENNQTD QDLGDNSRNL VGRGSSPRGS LSPRSPVSSL QIRYDQPGNS
721SLENLPPVAA SIEQLLERQW SEGQQFLLEQ GTPSDILGML KSLHQLQVEN RRLEEQIKNL
781TAKKERLQLL NAQLSVPFPT ITANPSPSHQ IHTFSAQTAP TTDSLNSSKS PHIGNSFLPD
841NSLPVLNQDL TSSGQSTSSS SALSTPPPAG QSPAQQGSGV SGVQQVNGVT VGALASGMQP
901VTSTIPAVSA VGGIIGALPG NQLAINGIVG ALNGVMQTPV TMSQNPTPLT HTTVPPNATH
961PMPATLTNSA SGLGLLSDQQ RQILIHQQQF QQLLNSQQLT PVHRHPHFTQ LPPTHFSPSM
1021EIMQVRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1027 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.