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MKRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MKRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114245-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MKRN1 (NM_013446), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Makorin ring finger protein 1 | MKRN1 | RNF61 | RING finger protein 61
Origin Species
Human
NCBI GenBank
NM_013446
SwissProt
Q9UHC7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MAEAATPGTT ATTSGAGAAA ATAAAASPTP IPTVTAPSLG AGGGGGGSDG SGGGWTKQVT
61CRYFMHGVCK EGDNCRYSHD LSDSPYSVVC KYFQRGYCIY GDRCRYEHSK PLKQEEATAT
121ELTTKSSLAA SSSLSSIVGP LVEMNTGEAE SRNSNFATVG AGSEDWVNAI EFVPGQPYCG
181RTAPSCTEAP LQGSVTKEES EKEQTAVETK KQLCPYAAVG ECRYGENCVY LHGDSCDMCG
241LQLLHPMDAA QRSQHIKSCI EAHEKDMELS FAVQRSKDMV CGICMEVVYE KANPSERRFG
301ILSNCNHTYC LKCIRKWRSA KQFESKIIKS CPECRITSNF VIPSEYWVEE KEEKQKLILK
361YKEAMSNKAC RYFDEGRGSC PFGGNCFYKH AYPDGRREEP QRQKVGTSSR YRAQRRNHFW
421ELIEERENSN PFDNDEEEVV TFELGEMLLM LLAAGGDDEL TDSEDEWDLF HDELEDFYDL
481DL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

482 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.