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MKL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MKL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114174-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MKL2 (NM_014048), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MKL/myocardin-like protein 2 | MKL/myocardin-like 2 | Megakaryoblastic leukemia 2 | NPD001 | KIAA1243 | MRTF-B | MRTFB
Origin Species
Human
NCBI GenBank
NM_014048
SwissProt
Q9ULH7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDHTGAIDTE DEVGPLAHLA PSPQSEAVAH EFQELSLQSS QNLPPLNERK NVLQLRLQQR
61RTREQLVDQG IMPPLKSPAA FHEQIKSLER ARTENFLKHK IRSRPDRSEL VRMHILEETF
121AEPSLQATQM KLKRARLADD LNEKIAQRPG PMELVEKNIL PVDSSVKEAI IGVGKEDYPH
181TQGDFSFDED SSDALSPDQP ASQESQGSAA SPSEPKVSES PSPVTTNTPA QFASVSPTVP
241EFLKTPPTAD QPPPRPAAPV LPTNTVSSAK PGPALVKQSH PKNPNDKHRS KKCKDPKPRV
301KKLKYHQYIP PDQKGEKNEP QMDSNYARLL QQQQLFLQLQ ILSQQKQHYN YQTILPAPFK
361PLNDKNSNSG NSALNNATPN TPRQNTSTPV RKPGPLPSSL DDLKVSELKT ELKLRGLPVS
421GTKPDLIERL KPYQEVNSSG LAAGGIVAVS SSAIVTSNPE VTVALPVTTL HNTVTSSVST
481LKAELPPTGT SNATRVENVH SPLPISPSPS EQSSLSTDDT NMADTFTEIM TMMSPSQFLS
541SSPLRMTNNE DSLSPTSSTL SNLELDAAEK DRKLQEKEKQ IEELKRKLEQ EQKLVEVLKM
601QLEVEKRGQQ QRPLEAQPSA PGHSVKSDQK HGSLGSSIKD EASLPDCSSS RQPIPVASHA
661VGQPVSTGGQ TLVAKKAVVI KQEVPVGQAE QQSVVSQFYT SPQAGMQTQP QIATAAQIPT
721AALASGLAPT VPQTQDTFPQ HVLSQPQQVR KVFTNSASSN TVLPYQRHPA PAVQQPFINK
781ASNSVLQSRN APLPSLQNGP NTPNKPSSPP PPQQFVVQHS LFGSPVAKTK DPPRYEEAIK
841QTRSTQAPLP EISNAHSQQM DDLFDILIKS GEISLPIKEE PSPISKMRPV TASITTMPVN
901TVVSRPPPQV QMAPPVSLEP MGSLSASLEN QLEAFLDGTL PSANEIPPLQ SSSEDREPFS
961LIEDLQNDLL SHSGMLDHSH SPMETSETQF AAGTPCLSLD LSDSNLDNME WLDITMPNSS
1021SGLTPLSTTA PSMFSADFLD PQDLPLPWD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1049 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.