Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MISP / C19orf21 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105329-100
Catalog Number: LS-G105329
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C19orf21(MISP) (NM_173481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C19orf21
- Origin Species
- Human
- NCBI GenBank
- NM_173481
- SwissProt
- Q8IVT2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRVTRYPIL GIPQAHRGTG LVLDGDTSYT YHLVCMGPEA SGWGQDEPQT WPTDHRAQQG61VQRQGVSYSV HAYTGQPSPR GLHSENREDE GWQVYRLGAR DAHQGRPTWA LRPEDGEDKE121MKTYRLDAGD ADPRRLCDLE RERWAVIQGQ AVRKSSTVAT LQGTPDHGDP RTPGPPRSTP181LEENVVDREQ IDFLAARQQF LSLEQANKGA PHSSPARGTP AGTTPGASQA PKAFNKPHLA241NGHVVPIKPQ VKGVVREENK VRAVPTWASV QVVDDPGSLA SVESPGTPKE TPIEREIRLA301QEREADLREQ RGLRQATDHQ ELVEIPTRPL LTKLSLITAP RRERGRPSLY VQRDIVQETQ361REEDHRREGL HVGRASTPDW VSEGPQPGLR RALSSDSILS PAPDARAADP APEVRKVNRI421PPDAYQPYLS PGTPQLEFSA FGAFGKPSSL STAEAKAATS PKATMSPRHL SESSGKPLST481KQEASKPPRG CPQANRGVVR WEYFRLRPLR FRAPDEPQQA QVPHVWGWEV AGAPALRLQK541SQSSDLLERE RESVLRREQE VAEERRNALF PEVFSPTPDE NSDQNSRSSS QASGITGSYS601VSESPFFSPI HLHSNVAWTV EDPVDSAPPG QRKKEQWYAG INPSDGINSE VLEAIRVTRH661KNAMAERWES RIYASEEDDT RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
710 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.