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MIPOL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MIPOL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107248-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIPOL 1(MIPOL1) (NM_138731), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MIPOL1 | Mirror-image polydactyly 1
Origin Species
Human
SwissProt
Q8TD10

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENWSKDITH SYLEQETTGI NKSTQPDEQL TMNSEKSMHR KSTELVNEIT CENTEWPGQR
61STNFQIISSY PDDESVYCTT EKYNVMEHRH NDMHYECMTP CQVTSDSDKE KTIAFLLKEL
121DILRTSNKKL QQKLAKEDKE QRKLKFKLEL QEKETEAKIA EKTAALVEEV YFAQKERDEA
181VMSRLQLAIE ERDEAIARAK HMEMSLKVLE NINPEENDMT LQELLNRINN ADTGIAIQKN
241GAIIVDRIYK TKECKMRITA EEMSALIEER DAALSKCKRL EQELHHVKEQ NQTSANNMRH
301LTAENNQERA LKAKLLSMQQ ARETAVQQYK KLEEEIQTLR VYYSLHKSLS QEENLKDQFN
361YTLSTYEEAL KNRENIVSIT QQQNEELATQ LQQALTERAN MELQLQHARE ASQVANEKVQ
421KLERLVDVLR KKVGTGTMRT VI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

442 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.