Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MIOS / FLJ20323 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111523-100
Catalog Number: LS-G111523
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIOS (NM_019005), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLJ20323 | MIOS | WUGSC:H_DJ1159O04.1 | H_DJ1159O04.1
- Origin Species
- Human
- NCBI GenBank
- NM_019005
- SwissProt
- Q9NXC5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGTKPDILW APHHVDRFVV CDSELSLYHV ESTVNSELKA GSLRLSEDSA ATLLSINSDT61PYMKCVAWYL NYDPECLLAV GQANGRVVLT SLGQDHNSKF KDLIGKEFVP KHARQCNTLA121WNPLDSNWLA AGLDKHRADF SVLIWDICSK YTPDIVPMEK VKLSAGETET TLLVTKPLYE181LGQNDACLSL CWLPRDQKLL LAGMHRNLAI FDLRNTSQKM FVNTKAVQGV TVDPYFHDRV241ASFYEGQVAI WDLRKFEKPV LTLTEQPKPL TKVAWCPTRT GLLATLTRDS NIIRLYDMQH301TPTPIGDETE PTIIERSVQP CDNYIASFAW HPTSQNRMIV VTPNRTMSDF TVFERISLAW361SPITSLMWAC GRHLYECTEE ENDNSLEKDI ATKMRLRALS RYGLDTEQVW RNHILAGNED421PQLKSLWYTL HFMKQYTEDM DQKSPGNKGS LVYAGIKSIV KSSLGMVESS RHNWSGLDKQ481SDIQNLNEER ILALQLCGWI KKGTDVDVGP FLNSLVQEGE WERAAAVALF NLDIRRAIQI541LNEGASSEKG DLNLNVVAMA LSGYTDEKNS LWREMCSTLR LQLNNPYLCV MFAFLTSETG601SYDGVLYENK VAVRDRVAFA CKFLSDTQLN RYIEKLTNEM KEAGNLEGIL LTGLTKDGVD661LMESYVDRTG DVQTASYCML QGSPLDVLKD ERVQYWIENY RNLLDAWRFW HKRAEFDIHR721SKLDPSSKPL AQVFVSCNFC GKSISYSCSA VPHQGRGFSQ YGVSGSPTKS KVTSCPGCRK781PLPRCALCLI NMGTPVSSCP GGTKSDEKVD LSKDKKLAQF NNWFTWCHNC RHGGHAGHML841SWFRDHAECP VSACTCKCMQ LDTTGNLVPA ETVQPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
875 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.