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MIER3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MIER3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MIER3 antibodies →
$504.00each
Catalog Number: LS-G106151-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIER3 (NM_152622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Mi-er3 | MIER3
Origin Species
Human
NCBI GenBank
NM_152622
SwissProt
Q7Z3K6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLVHDYDDER TLEEEEMMDE GKNFSSEIED LEKEGTMPLE DLLAFYGYEP TIPAVANSSA
61NSSPSELADE LPDMTLDKEE IAKDLLSGDD EETQSSADGL TPSVTSHETS DFFPRPLRSN
121TACDGDKESE VEDVETDSGN SPEDLRKEIM IGLQYQAEIP PYLGEYDGNE KVYENEDQLL
181WCPDVVLESK VKEYLVETSL RTGSEKIMDR ISAGTHTRDN EQALYELLKC NHNIKEAIER
241YCCNGKASQG MTAWTEEECR SFEHALMLFG KDFHLIQKNK VRTRTVAECV AFYYMWKKSE
301RYDYFAQQTR FGKKRYNHHP GVTDYMDRLV DETEALGGTV NASALTSNRP EPIPDQQLNI
361LNSFTASDLT ALTNSVATVC DPTDVNCLDD SFPPLGNTPR GQVNHVPVVT EELLTLPSNG
421ESDCFNLFET GFYHSELNPM NMCSEESERP AKRLKMGIAV PESFMNEVSV NNLGVDFENH
481THHITSAKMA VSVADFGSLS ANETNGFISA HALHQHAALH SE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.