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MIDN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MIDN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MIDN antibodies →
$504.00each
Catalog Number: LS-G104893-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIDN (NM_177401), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Midbrain nucleolar protein | MIDN | Midnolin
Origin Species
Human
NCBI GenBank
NM_177401
SwissProt
Q504T8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPQPGGARS CRRGAPGGAC ELGPAAEAAP MSLAIHSTTG TRYDLAVPPD ETVEGLRKRL
61SQRLKVPKER LALLHKDTRL SSGKLQEFGV GDGSKLTLVP TVEAGLMSQA SRPEQSVMQA
121LESLTETQVS DFLSGRSPLT LALRVGDHMM FVQLQLAAQH APLQHRHVLA AAAAAAAARG
181DPSIASPVSS PCRPVSSAAR VPPVPTSPSP ASPSPITAGS FRSHAASTTC PEQMDCSPTA
241SSSASPGAST TSTPGASPAP RSRKPGAVIE SFVNHAPGVF SGTFSGTLHP NCQDSSGRPR
301RDIGTILQIL NDLLSATRHY QGMPPSLAQL RCHAQCSPAS PAPDLAPRTT SCEKLTAAPS
361ASLLQGQSQI RMCKPPGDRL RQTENRATRC KVERLQLLLQ QKRLRRKARR DARGPYHWSP
421SRKAGRSDSS SSGGGGSPSE ASGLGLDFED SVWKPEVNPD IKSEFVVA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

468 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.