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MICALCL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MICALCL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MICALCL antibodies →
$725.00each
Catalog Number: LS-G108596-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MICALCL (NM_032867), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ebitein1 | Ebitein-1 | MICAL C-terminal like | MICAL C-terminal-like protein | MICALCL
Origin Species
Human
NCBI GenBank
NM_032867
SwissProt
Q6ZW33

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSPPKDPSPS LPLPSSSSHS SSPPSSSSTS VSGNAPDGSS PPQMTASEPL SQVSRGHPSP
61PTPNFRRRAV AQGAPREIPL YLPHHPKPEW AEYCLVSPGE DGLSDPAEMT SDECQPAEAP
121LGDIGSNHRD PHPIWGKDRS WTGQELSPLA GEDREKGSTG ARKEEEGGPV LVKEKLGLKK
181LVLTQEQKTM LLDWNDSIPE SVHLKAGERI SQKSAENGRG GRVLKPVRPL LLPRAAGEPL
241PTQRGAQEKM GTPAEQAQGE RNVPPPKSPL RLIANAIRRS LEPLLSNSEG GKKAWAKQES
301KTLPAQACTR SFSLRKTNSN KDGDQHSPGR NQSSAFSPPD PALRTHSLPN RPSKVFPALR
361SPPCSKIEDV PTLLEKVSLQ ENFPDASKPP KKRISLFSSL RLKDKSFESF LQESRQRKDI
421RDLFGSPKRK VLPEDSAQAL EKLLQPFKST SLRQAAPPPP PPPPPPPPPP TAGGADSKNF
481PLRAQVTEAS SSASSTSSSS ADEEFDPQLS LQLKEKKTLR RRKKLEKAMK QLVKQEELKR
541LYKAQAIQRQ LEEVEERQRA SEIQGVRLEK ALRGEADSGT QDEAQLLQEW FKLVLEKNKL
601MRYESELLIM AQELELEDHQ SRLEQKLREK MLKEESQKDE KDLNEEQEVF TELMQVIEQR
661DKLVDSLEEQ RIREKAEDQH FESFVFSRGC QLSRT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

695 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.