Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MICAL1 / MICAL Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128818-100
Catalog Number: LS-G128818
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MICAL1 (NM_001159291), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MICAL | MICAL1 | NICAL | CasL interacting molecule | MICAL-1
- Origin Species
- Human
- NCBI GenBank
- NM_022765
- SwissProt
- Q8TDZ2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASPTSTNPA HAHFESFLQA QLCQDVLSSF QELCGALGLE PGGGLPQYHK IKDQLNYWSA61KSLWTKLDKR AGQPVYQQGR ACTSTKCLVV GAGPCGLRVA VELALLGARV VLVEKRTKFS121RHNVLHLWPF TIHDLRALGA KKFYGRFCTG TLDHISIRQL QLLLLKVALL LGVEIHWGVT181FTGLQPPPRK GSGWRAQLQP NPPAQLANYE FDVLISAAGG KFVPEGFKVR EMRGKLAIGI241TANFVNGRTV EETQVPEISG VARIYNQSFF QSLLKATGID LENIVYYKDD THYFVMTAKK301QCLLRLGVLR QPFWPLGTGV ARGFLAAFDA AWMVKRWAEG AESLEVLAER ESLYQLLSQT361SPENMHRNVA QYGLDPATRY PNLNLRAVTP NQVRDLYDVL AKEPVQRNND KTDTGMPATG421SAGTQEELLR WCQEQTAGYP GVHVSDLSSS WADGLALCAL VYRLQPGLLE PSELQGLGAL481EATAWALKVA ENELGITPVV SAQAVVAGSD PLGLIAYLSH FHSAFKSMAH SPGPVSQASP541GTSSAVLFLS KLQRTLQRSR AKENAEDAGG KKLRLEMEAE TPSTEVPPDP EPGVPLTPPS601QHQEAGAGDL CALCGEHLYV LERLCVNGHF FHRSCFRCHT CEATLWPGGY EQHPGDGHFY661CLQHLPQTDH KAEGSDRGPE SPELPTPSEN SMPPGLSTPT ASQEGAGPVP DPSQPTRRQI721RLSSPERQRL SSLNLTPDPE MEPPPKPPRS CSALARHALE SSFVGWGLPV QSPQALVAME781KEEKESPFSS EEEEEDVPLD SDVEQALQTF AKTSGTMNNY PTWRRTLLRR AKEEEMKRFC841KAQTIQRRLN EIEAALRELE AEGVKLELAL RRQSSSPEQQ KKLWVGQLLQ LVDKKNSLVA901EEAELMITVQ ELNLEEKQWQ LDQELRGYMN REENLKTAAD RQAEDQVLRK LVDLVNQRDA961LIRFQEERRL SELALGTGAQ GTRTRPLEQKLISEEDLAANDILDYKDDDDKV
981 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.