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MIB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MIB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MIB2 antibodies →
$725.00each
Catalog Number: LS-G130372-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MIB2(MIB2) (NM_001170686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MIB2 | Mindbomb homolog 2 | Novel zinc finger protein | Mind bomb homolog 2 | ZZANK1 | SKD | Skeletrophin | ZZZ5 | Novelzin
Origin Species
Human
SwissProt
Q96AX9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MAGALRRGRA LGSRPSGPTV SSRRSPQCPV AQEGLGARSR PRVAPRSLAR CGPSSRLMGW
61KPSEARGQSQ SFQASGLQPR SLKAARRATG RPDRSRAARP TMDPSAHRSR AAPPNMDPDP
121QAGVQVGMRV VRGVDWKWGQ QDGGEGGVGT VVELGRHGSP STPDRTVVVQ WDQGTRTNYR
181AGYQGAHDLL LYDNAQIGVR HPNIICDCCK KHGLRGMRWK CRVCLDYDLC TQCYMHNKHE
241LAHAFDRYET AHSRPVTLSP RQGLPRIPLR GIFQGAKVVR GPDWEWGSQD GGEGKPGRVV
301DIRGWDVETG RSVASVTWAD GTTNVYRVGH KGKVDLKCVG EAAGGFYYKD HLPRLGKPAE
361LQRRVSADSQ PFQHGDKVKC LLDTDVLREM QEGHGGWNPR MAETGTVHRI TDRGDVRVQF
421NHETRWTFHP GALTKHHSFW VGDVVRVIGD LDTVKRLQAG HGEWTDDMAP ALGRVGKVVK
481VFGDGNLRVA VAGQRWTFSP SCLVAYRPEE DANLDVAERA RENKSSLSVA LDKLRAQKSD
541PEHPGRLVVE VALGNAARAL DLLRRRPEQV DTKNQGRTAL QVAAYLGQVE LIRLLLQARA
601GVDLPDDEGN TALHYAALGN QPEATRVLLS AGCRADAINS TQSTALHVAV QRGFLEVVRA
661LCERGCDVNL PDAHSDTPLH SAISAGTGAS GIVEVLTEVP NIDVTATNSQ GFTLLHHASL
721KGHALAVRKI LARARQLVDA KKEDGFTALH LAALNNHREV AQILIREGRC DVNVRNRKLQ
781SPLHLAVQQA HVGLVPLLVD AGCSVNAEDE EGDTALHVAL QRHQLLPLVA DGAGGDPGPL
841QLLSRLQASG LPGSAELTVG AAVACFLALE GADVSYTNHR GRSPLDLAAE GRVLKALQGC
901AQRFRERQAG GGAAPGPRQT LGTPNTVTNL HVGAAPGPEA AECLVCSELA LLVLFSPCQH
961RTVCEECARR MKKCIRCQVV VSKKLRPDGS EVASAAPAPG PPRQLVEELQ SRYRQMEERI
1021TCPICIDSHI RLVFQCGHGA CAPCGSALSA CPICRQPIRD RIQIFV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1066 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.