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MGRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MGRN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113382-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF156(MGRN1) (NM_015246), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0544 | Mahogunin | ring finger 1 | MGRN1 | RNF156 | RING finger protein 156
Origin Species
Human
NCBI GenBank
AB011116
SwissProt
O60291

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSILSRRIA GVEDIDIQAN SAYRYPPKSG NYFASHFFMG GEKFDTPHPE GYLFGENMDL
61NFLGSRPVQF PYVTPAPHEP VKTLRSLVNI RKDSLRLVRY KDDADSPTED GDKPRVLYSL
121EFTFDADARV AITIYCQASE EFLNGRAVYS PKSPSLQSET VHYKRGVSQQ FSLPSFKIDF
181SEWKDDELNF DLDRGVFPVV IQAVVDEGDV VEVTGHAHVL LAAFEKHMDG SFSVKPLKQK
241QIVDRVSYLL QEIYGIENKN NQETKPSDDE NSDNSNECVV CLSDLRDTLI LPCRHLCLCT
301SCADTLRYQA NNCPICRLPF RALLQIRAVR KKPGALSPVS FSPVLAQSLE HDEHSCPFKK
361SKPHPASLAS KKPKRETNSD SVPPGYEPIS LLEALNGLRA VSPAIPSAPL YEEITYSGIS
421DGLSQASCPL AAIDHILDSS RQKGRPQSKA PDSTLRSPSS PIHEEDEEKL SEDVDAPPPL
481GGAELALRES SSPESFITEE VDESSSPQQG TRAASIENVL QDSSPEHCGR GPPADIYLPG
541RPTSMETAHG LATTSPTWPP LGGPSPDPSA AELTPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

576 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.