LSBio
MGC50722 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MGC50722 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MGC50722 antibodies →
$504.00each
Catalog Number: LS-G103181-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MGC50722 (NM_203348), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MGC50722
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTLVVGTPP TCLGDTPQPC HKNSQRQGPF SHGAPGRAAD WKAVAKPRLC APAAEDDVAA
61LRWPGPSQQP DPPWAAPHVV GSDDLKEPGP WGKACSLPMW STGPEARDGD SSVSSGRLSC
121SSGGHDVCVS WKERPPQVLG PQQRPRKSDA RLEQLRDKIR AQAWQQGSCA SLGTSAPSSA
181SRLHKASTLT LRRKGQEAKN PPPAPECSGF SILSAAERRV EAKASHGQGR ELSRVSQHQV
241PVLREKPKRV KSSSCKREKT PKLPSPRRAA KDKHKDEDSE LVGVYAWRKG QALVRSLLGP
301PPVLRRHHSK DPSRDPALTV DLGDSEKVIA AKCSPVCAQL PDATSAHSDQ QVSGNTPSLA
361SFDQPATIQT AMAILQDLRQ QIQAGLELAQ ARKGGQELGP SKRRLQDVAG RGRCRDPNAQ
421SSFSKSPWAM TERKHSSLER ARSVHTWEPW SSSTARESCP QRAWGAQGQD RSFQRPESPH
481ERLGHFSQRP WSALAGQACS PQRAWGAQRQ GPSSQRPGSP PEKRSPFPQQ PWSAVATQPC
541PRRAWTACET WEDPGPRLRN PLERPSPPAQ RPWSSSGVQR AGPQGKGRGI GSPVSAAKHA
601LPRPTGSFPQ NPLGKEKDTL RPCPRSRGLL GPSHSSESLR EFMRQKAQAR RRQALEEKAS
661ALRTRELRSR RLQEVYRQQR EAVLGRAVPV VSRTTPGIVT FVPSSAQSGG LEASGSLESP
721VLEWSKVTSG MVLGGQEAPG SFCLCLNRAW NHAETLDPPG MGGPQDGRDA PVLLSASPSL
781GSLELQDLTT RYLPRGMCIY LDPKEAEHLG TSSSLHLRHK QAQLQALETT AKVLKQRVDS
841LTAKLQGAEA PDTVRDPAVG LLRSCPHSLP AAPTLATPTL ATPACPGALG PNWGRGAPGE
901WVSMQPQPLL PPTYFLEGET LSWGPSWEQQ QSVSPRAHCE SKPRGVSQAP LLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

953 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.