Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MGC11296 / BRMS1L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108885-100
Catalog Number: LS-G108885
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRMS1L (NM_032352), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BRMS1-homolog protein p40 | BRMS1L | MGC11296 | BRMS1-like protein p40
- Origin Species
- Human
- NCBI GenBank
- NM_032352
- SwissProt
- Q5PSV4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPVHSRGDKK ETNHHDEMEV DYAENEGSSS EDEDTESSSV SEDGDSSEMD DEDCERRRME61CLDEMSNLEK QFTDLKDQLY KERLSQVDAK LQEVIAGKAP EYLEPLATLQ ENMQIRTKVA121GIYRELCLES VKNKYECEIQ ASRQHCESEK LLLYDTVQSE LEEKIRRLEE DRHSIDITSE181LWNDELQSRK KRKDPFSPDK KKPVVVSGPY IVYMLQDLDI LEDWTTIRKA MATLGPHRVK241TEPPVKLEKH LHSARSEEGR LYYDGEWYIR GQTICIDKKD ECPTSAVITT INHDEVWFKR301PDGSKSKLYI SQLQKGKYSI KHSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
323 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.