Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MGAT / GLYT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99742-100
Catalog Number: LS-G99742
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MGAT1 (NM_002406), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GGNT1 | GLCT1 | GLYT1 | GNT-1 | MGAT | MGAT1 | GlcNAc-T I | GLCNAC-TI | GNT-I
- NCBI GenBank
- NM_002406
- SwissProt
- P26572
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLKKQSAGLV LWGAILFVAW NALLLLFFWT RPAPGRPPSV SALDGDPASL TREVIRLAQD61AEVELERQRG LLQQIGDALS SQRGRVPTAA PPAQPRVPVT PAPAVIPILV IACDRSTVRR121CLDKLLHYRP SAELFPIIVS QDCGHEETAQ AIASYGSAVT HIRQPDLSSI AVPPDHRKFQ181GYYKIARHYR WALGQVFRQF RFPAAVVVED DLEVAPDFFE YFRATYPLLK ADPSLWCVSA241WNDNGKEQMV DASRPELLYR TDFFPGLGWL LLAELWAELE PKWPKAFWDD WMRRPEQRQG301RACIRPEISR TMTFGRKGVS HGQFFDQHLK FIKLNQQFVH FTQLDLSYLQ REAYDRDFLA361RVYGAPQLQV EKVRTNDRKE LGEVRVQYTG RDSFKAFAKA LGVMDDLKSG VPRAGYRGIV421TFQFRGRRVH LAPPPTWEGY DPSWNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.