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MFSD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MFSD7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MFSD7 antibodies →
$504.00each
Catalog Number: LS-G108973-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MFSD7 (NM_032219), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_032219
SwissProt
Q6UXD7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGPTEAETG LAEPRALCAQ RGHRTYARRW VFLLAISLLN CSNATLWLSF APVADVIAED
61LVLSMEQINW LSLVYLVVST PFGVAAIWIL DSVGLRAATI LGAWLNFAGS VLRMVPCMVV
121GTQNPFAFLM GGQSLCALAQ SLVIFSPAKL AALWFPEHQR ATANMLATMS NPLGVLVANV
181LSPVLVKKGE DIPLMLGVYT IPAGVVCLLS TICLWESVPP TPPSAGAASS TSEKFLDGLK
241LLMWNKAYVI LAVCLGGMIG ISASFSALLE QILCASGHSS GFSGLCGALF ITFGILGALA
301LGPYVDRTKH FTEATKIGLC LFSLACVPFA LVSQLQGQTL ALAATCSLLG LFGFSVGPVA
361MELAVECSFP VGEGAATGMI FVLGQAEGIL IMLAMTALTV RRSEPSLSTC QQGEDPLDWT
421VSLLLMAGLC TFFSCILAVF FHTPYRRLQA ESGEPPSTRN AVGGADSGPG VDRGGAGRAG
481VLGPSTATPE CTARGASLED PRGPGSPHPA CHRATPRAQG PAATDAPSRP GRLAGRVQAS
541RFIDPAGSHS SFSSPWVIT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

559 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.