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MFSD12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MFSD12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MFSD12 antibodies →
$725.00each
Catalog Number: LS-G110635-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C19orf28(MFSD12) (NM_021731), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_021731
SwissProt
Q6NUT3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPGPPAAGA APSPRPLSLV ARLSYAVGHF LNDLCASMWF TYLLLYLHSV RAYSSRGAGL
61LLLLGQVADG LCTPLVGYEA DRAASCCARY GPRKAWHLVG TVCVLLSFPF IFSPCLGCGA
121ATPEWAALLY YGPFIVIFQF GWASTQISHL SLIPELVTND HEKVELTALR YAFTVVANIT
181VYGAAWLLLH LQGSSRVEPT QDISISDQLG GQDVPVFRNL SLLVVGVGAV FSLLFHLGTR
241ERRRPHAEEP GEHTPLLAPA TAQPLLLWKH WLREPAFYQV GILYMTTRLI VNLSQTYMAM
301YLTYSLHLPK KFIATIPLVM YLSGFLSSFL MKPINKCIGR NMTYFSGLLV ILAFAAWVAL
361AEGLGVAVYA AAVLLGAGCA TILVTSLAMT ADLIGPHTNS GAFVYGSMSF LDKVANGLAV
421MAIQSLHPCP SELCCRACVS FYHWAMVAVT GGVGVAAALC LCSLLLWPTR LRRSFLAWRR
481GRGEDKGPGY SWIPTVLVQP PRPTSPFLWE APLAWRCTRK KWSGAGTKPW PREDWGTH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

538 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.