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MFHAS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MFHAS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MFHAS1 antibodies →
$725.00each
Catalog Number: LS-G116791-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Malignant fibrous histiocytoma(MFHAS1) (NM_004225), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MASL1 | LRRC65 | MFHAS1
Origin Species
Human
NCBI GenBank
NM_004225
SwissProt
Q9Y4C4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGMDSGNLK TARLWRDAAL RARKLRSNLR QLTLTAAGAC PGAGADALES PASPQLVLPA
61NLGDIEALNL GNNGLEEVPE GLGSALGSLR VLVLRRNRFA RLPPAVAELG HHLTELDVSH
121NRLTALGAEV VSALRELRKL NLSHNQLPAL PAQLGALAHL EELDVSFNRL AHLPDSLSCL
181SRLRTLDVDH NQLTAFPRQL LQLVALEELD VSSNRLRGLP EDISALRALK ILWLSGAELG
241TLPAGFCELA SLESLMLDNN GLQALPAQFS CLQRLKMLNL SSNLFEEFPA ALLPLAGLEE
301LYLSRNQLTS VPSLISGLGR LLTLWLDNNR IRYLPDSIVE LTGLEELVLQ GNQIAVLPDH
361FGQLSRVGLW KIKDNPLIQP PYEVCMKGIP YIAAYQKELA HSQPAVQPRL KLLLMGHKAA
421GKTLLRHCLT EERVEGCPGG GDKEKCYPPS PPPVSKGIEV TSWTADASRG LRFIVYDLAG
481DESYEVIQPF FLSPGALYVL VVNLATYEPR HFPTTVGSFL HRVGARVPHA VVCIVGTHAD
541LCGERELEEK CLDIHRQIAL QEKHDAEGLS RLAKVVDEAL ARDFELRSAS PHAAYYGVSD
601KNLRRRKAHF QYLLNHRLQI LSPVLPVSCR DPRHLRRLRD KLLSVAEHRE IFPNLHRVLP
661RSWQVLEELH FQPPQAQRLW LSWWDSARLG LQAGLTEDRL QSALSYLHES GKLLYFEDSP
721ALKEHVFHNL TRLIDILNVF FQRDPSLLLH KLLLGTSGEG KAEGESSPPM ARSTPSQELL
781RATQLHQYVE GFLLHGLLPA HVIRLLLKPH VQAQQDLQLL LELLEKMGLC YCLNKPKGKP
841LNGSTAWYKF PCYVQNEVPH AEAWINGTNL AGQSFVAEQL QIEYSFPFTF PLGLFARYSV
901QINSHVVHRS DGKFQIFAYR GKVPVVVSYR PARGVLQPDT LSIASHASLP NIWTAWQAIT
961PLVEELNVLL QEWPGLHYTV HILCSKCLKR GSPNPHAFPG ELLSQPRPEG VAEIICPKNG
1021SERVNVALVY PPTPTVISPC SKKNVGEKHR NQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1052 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.