Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MFAP3L / NYD-Sp9 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110623-100
Catalog Number: LS-G110623
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MFAP3L (NM_021647), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0626 | MFAP3L | NYD-sp9
- Origin Species
- Human
- NCBI GenBank
- AF327560
- SwissProt
- O75121
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRLKSHLTV CFLPSVPFLI LVSTLATAKS VTNSTLNGTN VVLGSVPVII ARTDHIIVKE61GNSALINCSV YGIPDPQFKW YNSIGKLLKE EEDEKERGGG KWQMHDSGLL NITKVSFSDR121GKYTCVASNI YGTVNNTVTL RVIFTSGDMG VYYMVVCLVA FTIVMVLNIT RLCMMSSHLK181KTEKAINEFF RTEGAEKLQK AFEIAKRIPI ITSAKTLELA KVTQFKTMEF ARYIEELARS241VPLPPLIMNC RTIMEEIMEV VGLEEQGQNF VRHTPEGQEA ADRDEVYTIP NSLKRSDSPA301ADSDASSLHE QPQQIAIKVS VHPQSKKEHA DDQEGGQFEV KDVEETELSA EHSPETAEPS361TNVTSTELTS EEPTPVEVPD KVLPPAYLEA TEPAVTHDKN TCIIYESHVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
409 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.