Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MEX3B / RKHD3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108959-100
Catalog Number: LS-G108959
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RKHD3(MEX3B) (NM_032246), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA2009 | MEX-3B | RKHD3 | Mex-3 homolog B (C. elegans) | MEX3B | RING finger protein 195 | RNA-binding protein MEX3B | RNF195
- Origin Species
- Human
- NCBI GenBank
- AL136778
- SwissProt
- Q6ZN04
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSSLFADLE RNGSGGGGGG SSGGGETLDD QRALQLALDQ LSLLGLDSDE GASLYDSEPR61KKSVNMTECV PVPSSEHVAE IVGRQGCKIK ALRAKTNTYI KTPVRGEEPV FVVTGRKEDV121AMARREIISA AEHFSMIRAS RNKNTALNGA VPGPPNLPGQ TTIQVRVPYR VVGLVVGPKG181ATIKRIQQQT HTYIVTPSRD KEPVFEVTGM PENVDRAREE IEAHIALRTG GIIELTDEND241FHANGTDVGF DLHHGSGGSG PGSLWSKPTP SITPTPGRKP FSSYRNDSSS SLGSASTDSY301FGGGTSSSAA ATQRLADYSP PSPALSFAHN GNNNNNGNGY TYTAGGEASV PSPDGCPELQ361PTFDPAPAPP PGAPLIWAQF ERSPGGGPAA PVSSSCSSSA SSSASSSSVV FPGGGASAPS421NANLGLLVHR RLHPGTSCPR LSPPLHMAPG AGEHHLARRV RSDPGGGGLA YAAYANGLGA481QLPGLQPSDT SGSSSSSSSS SSSSSSSSGL RRKGSRDCSV CFESEVIAAL VPCGHNLFCM541ECANRICEKS EPECPVCHTA VTQAIRIFSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
569 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.