Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
METTL9 / PAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112917-100
Catalog Number: LS-G112917
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human METTL9 (NM_016025), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CGI-81 | DORA reverse strand protein 1 | DREV1 | METTL9 | PAP1 | Methyltransferase like 9 | DORA reverse strand protein | DREV | p53 activated protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_016025
- SwissProt
- Q9H1A3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.42 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSGPGGPAA AAGGRKENHQ WYVCNREKLC ESLQAVFVQS YLDQGTQIFL NNSIEKSGWL61FIQLYHSFVS SVFSLFMSRT SINGLLGRGS MFVFSPDQFQ RLLKINPDWK THRLLDLGAG121DGEVTKIMSP HFEEIYATEL SETMIWQLQK KKYRVLGINE WQNTGFQYDV ISCLNLLDRC181DQPLTLLKDI RSVLEPTRGR VILALVLPFH PYVENVGGKW EKPSEILEIK GQNWEEQVNS241LPEVFRKAGF VIEAFTRLPY LCEGDMYNDY YVLDDAVFVL KPVTRTRPLE QKLISEEDLA301ANDILDYKDD DDKV
314 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.