Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
METTL22 / C16orf68 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110032-100
Catalog Number: LS-G110032
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human METTL22 (NM_024109), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C16orf68 | LP8272 | Methyltransferase like 22 | METTL22
- Origin Species
- Human
- NCBI GenBank
- NM_024109
- SwissProt
- Q9BUU2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVQLAPAAAM DEVTFRSDTV LSDVHLYTPN HRHLMVRLNS VGQPVFLSQF KLLWSQDSWT61DSGAKGGSHR DVHTKEPPSA ETGSTGSPPG SGHGNEGFSL QAGTDTTGQE VAEAQLDEDG121DLDVVRRPRA ASDSNPAGPL RDKVHPMILA QEEDDVLGEE AQGSPHDIIR IEHTMATPLE181DVGKQVWRGA LLLADYILFR QDLFRGCTAL ELGAGTGLAS IIAATMARTV YCTDVGADLL241SMCQRNIALN SHLAATGGGI VRVKELDWLK DDLCTDPKVP FSWSQEEISD LYDHTTILFA301AEVFYDDDLT DAVFKTLSRL AHRLKNACTA ILSVEKRLNF TLRHLDVTCE AYDHFRSCLH361ALEQLADGKL RFVVEPVEAS FPQLLVYERL QQLELWKIIA EPVTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
404 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.