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METT11D1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

METT11D1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all METTL17 antibodies →
$725.00each
Catalog Number: LS-G120822-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human METT11D1(METTL17) (NM_001029991), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
False p73 target gene protein | METTL17 | Methyltransferase like 17 | METT11D1
Origin Species
Human
NCBI GenBank
NM_022734
SwissProt
Q9H7H0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAALKCLLT LGRWCPGLGV APQARALAAL VPGVTQVDNK SGFLQKRPHR QHPGILKLPH
61VRLPQALANG AQLLLLGSAG PTMENQVQTL TSYLWSRHLP VEPEELQRRA RHLEKKFLEN
121PDLSQTEEKL RGAVLHALRK TTYHWQELSY TEGLSLVYMA ARLDGGFAAV SRAFHEIRAR
181NPAFQPQTLM DFGSGTGSVT WAAHSIWGQS LREYMCVDRS AAMLVLAEKL LKGGSESGEP
241YIPGVFFRQF LPVSPKVQFD VVVSAFSLSE LPSKADRTEV VQTLWRKTGH FLVLVENGTK
301AGHSLLMDAR DLVLKGKEKS PLDPRPGFVF APCPHELPCP QLTNLACSFS QAYHPIPFSW
361NKKPKEEKFS MVILARGSPE EAHRWPRITQ PVLKRPRHVH CHLCCPDGHM QHAVLTARRH
421GRYGGCDQNQ WDVAGSCSPR QHLFPQGFVS LCPCQLLGRS FTCAYSVCVS SIYGSGSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

478 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.