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MEI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MEI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MEI1 antibodies →
$725.00each
Catalog Number: LS-G106239-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEI1 (NM_152513), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_152513

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVRQAATAG TPGPRREEEA ALLFERAHYR HDPRWLLPVT PRLCLACALE LLPDPGVSLV
61RKKHMLSCFQ DALVRHTSLV TQLVSQDQRV CIHFISVLFG LLCSMEDGSV TDLCIEVLIQ
121ITTQLKLEQT IRCLLDECHK ELCNMPSMRG SLATLTLLGK LVDAIPALAD ELVMEHGNLM
181EHLLRGLVYP SEGIQASVCY LYGKLYSSPV AAEMLSGHFR EKLFPLFLSI LDGAQTKELQ
241INCLGLLRQL LKYDLFVSMI MNQDGLGESA KNIEGSSGNT SLPLVLKKLL LSRDETLQVA
301SAHCITAVLV HSPAKHASAF IHADIPEFLF EHLSSSSEVL VWSSCNCLTL LVEEPLFFSK
361CHTVYGIEAV VRSLQGSLKM NNIELHKQGL LLFAEILTRQ PEEIKLFTSS AMCRDAGRAL
421QEAVSSPVLE VAAEALKATS AFLRKDHQST PPVQYGELQA LLEAMLNRCA EFSQTLLSRR
481PLGHASSRDS EKAILQRGKF LLSTLEGFRS ACRLAIEFQS EPSAQENPFT APSAKKEDTL
541EAFSEFLLSA CDSLCIPMVM RHLEQTTHPA LMEVFLSILH NLFVIVPHMK EKFSKKLASS
601SFIRLTLELK ARFCSGLSHS ALNQVCSNFL YYMCLNLLSA PEKTGPPSKE ELSAVSELLQ
661HGLPQISSRS PESLAFLSDR QYMEGAARQR QYCILLLFYL AYIHEDRFVS EAELFEAVQS
721FLLSLQDQGE RPPLVVFKAS IYLLAICQDK DNTLRETMVS AIRKFLEGIP DLQLVYTHHP
781LLLRFFLLYP ELMSRYGHRV LELWFFWEES SYEELDDVTS AGQPALPASL VVLFQLLRSI
841PSILLILLDL IYSSPVDTAH KVLISLRTFL RRNEDIQVGG LIRGHFLLIL QRLLVEHGAS
901PSGASGNLPL LLSLLSLMQL RNVSEQELDS VAMKLLHQVS KLCGKCSPTD VDILQPSFNF
961LYWSLHQTTP SSQKRAAAVL LSSTGLMELL EKMLALTLAK ADSPRTALLC SAWLLTASFS
1021AQQHKGSLQV HQTLSVEMDQ VLKALSFPKK KAALLSAAIL CFLRTALRQS FSSALVALVP
1081SGAQPLPATK DTVLAPLRMS QVRSLVIGLQ NLLVQKDPLL SQACVGCLEA LLDYLDARSP
1141DIALHVASQP WNRFLLFTLL DAGENSFLRP EILRLMTLFM RYRSSSVLSH EEVGDVLQGV
1201ALADLSTLSN TTLQALHGFF QQLQSMGHLA DHSMAQTLQA SLEGLPPSTS SGQPPLQDML
1261CLGGVAVSLS HIRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1274 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.