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MEGF6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MEGF6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118602-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEGF6 (NM_001409), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EGF-like protein 3 | EGFL3 | EGF-like-domain | multiple 3 | MEGF6 | Multiple EGF-like-domains 6 | KIAA0815
Origin Species
Human
NCBI GenBank
AF086226

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
161.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSFLEEARAA GRAVVLALVL LLLPAVPVGA SVPPRPLLPL QPGMPHVCAE QELTLVGRRQ
61PCVQALSHTV PVWKAGCGWQ AWCVGHERRT VYYMGYRQVY TTEARTVLRC CRGWMQQPDE
121EGCLSAECSA SLCFHGGRCV PGSAQPCHCP PGFQGPRCQY DVDECRTHNG GCQHRCVNTP
181GSYLCECKPG FRLHTDSRTC LAINSCALGN GGCQHHCVQL TITRHRCQCR PGFQLQEDGR
241HCVRRSPCAN RNGSCMHRCQ VVRGLARCEC HVGYQLAADG KACEDVDECA AGLAQCAHGC
301LNTQGSFKCV CHAGYELGAD GRQCYRIEME IVNSCEANNG GCSHGCSHTS AGPLCTCPRG
361YELDTDQRTC IDVDDCADSP CCQQVCTNNP GGYECGCYAG YRLSADGCGC EDVDECASSR
421GGCEHHCTNL AGSFQCSCEA GYRLHEDRRG CSPLEEPMVD LDGELPFVRP LPHIAVLQDE
481LPQLFQDDDV GADEEEAELR GEHTLTEKFV CLDDSFGHDC SLTCDDCRNG GTCLLGLDGC
541DCPEGWTGLI CNETCPPDTF GKNCSFSCSC QNGGTCDSVT GACRCPPGVS GTNCEDGCPK
601GYYGKHCRKK CNCANRGRCH RLYGACLCDP GLYGRFCHLT CPPWAFGPGC SEECQCVQPH
661TQSCDKRDGS CSCKAGFRGE RCQAECELGY FGPGCWQACT CPVGVACDSV SGECGKRCPA
721GFQGEDCGQE CPVGTFGVNC SSSCSCGGAP CHGVTGQCRC PPGRTGEDCE ADCPEGRWGL
781GCQEICPACQ HAARCDPETG ACLCLPGFVG SRCQDVCPAG WYGPSCQTRC SCANDGHCHP
841ATGHCSCAPG WTGFSCQRAC DTGHWGPDCS HPCNCSAGHG SCDAISGLCL CEAGYVGPRC
901EQQCPQGHFG PGCEQRCQCQ HGAACDHVSG ACTCPAGWRG TFCEHACPAG FFGLDCRSAC
961NCTAGAACDA VNGSCLCPAG RRGPRCAETC PAHTYGHNCS QACACFNGAS CDPVHGQCHC
1021APGWMGPSCL QACPAGLYGD NCRHSCLCQN GGTCDPVSGH CACPEGWAGL ACEKECLPRD
1081VRAGCRHSGG CLNGGLCDPH TGRCLCPAGW TGDKCQSPCL RGWFGEACAQ RCSCPPGAAC
1141HHVTGACRCP PGFTGSGCEQ ACPPGSFGED CAQMCQCPGE NPACHPATGT CSCAAGYHGP
1201SCQQRCPPGR YGPGCEQLCG CLNGGSCDAA TGACRCPTGF LGTDCNLTCP QGRFGPNCTH
1261VCGCGQGAAC DPVTGTCLCP PGRAGVRCER GCPQNRFGVG CEHTCSCRNG GLCHASNGSC
1321SCGLGWTGRH CELACPPGRY GAACHLECSC HNNSTCEPAT GTCRCGPGFY GQACEHPCPP
1381GFHGAGCQGL CWCQHGAPCD PISGRCLCPA GFHGHFCERG CEPGSFGEGC HQRCDCDGGA
1441PCDPVTGLCL CPPGRSGATC NLDCRRGQFG PSCTLHCDCG GGADCDPVSG QCHCVDGYMG
1501PTCREGGPLR LPENPSLAQG SAGTLPASSR PTSRSGGPAR H
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1541 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.