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MEF2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MEF2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115646-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MEF2B(BORCS8-MEF2B) (NM_005919), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LOC729991-MEF2B readthrough | MEF2B neighbor gene protein | Myocyte enhancer factor 2B | LOC729991-MEF2B | MEF2BNB-MEF2B | MEF2BNB-MEF2B readthrough
Origin Species
Human
NCBI GenBank
NM_001145785

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MGRKKIQISR ILDQRNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS ANRLFQYAST
61DMDRVLLKYT EYSEPHESRT NTDILETLKR RGIGLDGPEL EPDEGPEEPG EKFRRLAGEG
121GDPALPRPRL YPAAPAMPSP DVVYGALPPP GCDPSGLGEA LPAQSRPSPF RPAAPKAGPP
181GLVHPLFSPS HLTSKTPPPL YLPTEGRRSD LPGGLAGPRG GLNTSRSLYS GLQNPCSTAT
241PGPPLGSFPF LPGGPPVGAE AWARRVPQPA APPRRPPQSA SSLSASLRPP GAPATFLRPS
301PIPCSSPGPW QSLCGLGPPC AGCPWPTAGP GRRSPGGTSP ERSPGTARAR GDPTSLQASS
361EKTQQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

365 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.