Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MED15 / ARC105 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101357-100
Catalog Number: LS-G101357
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MED15(MED15) (NM_015889), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAG7A | CTG repeat protein 7a | CTG7A | MED15 | Mediator complex subunit 15 | TIG1 | PCQAP | ARC105 | TIG-1 | TNRC7 | TPA inducible gene-1 | TPA inducible protein | TPA-inducible gene 1 protein
- Origin Species
- Human
- NCBI GenBank
- NM_015889
- SwissProt
- Q96RN5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 82.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA61RLIIHFRDIH NKKSQASVSD PMNALQSLTG GPAAGAAGIG MPPRGPGQSL GGMGSLGAMG121QPMSLSGQPP PGTSGMAPHS MAVVSTATPQ TQLQLQQVAL QQQQQQQQFQ QQQQAALQQQ181QQQQQQQQFQ AQQSAMQQQF QAVVQQQQQL QQQQQQQQHL IKLHHQNQQQ IQQQQQQLQR241IAQLQLQQQQ QQQQQQQQQQ QQALQAQPPI QQPPMQQPQP PPSQALPQQL QQMHHTQHHQ301PPPQPQQPPV AQNQPSQLPP QSQTQPLVSQ AQALPGQMLY TQPPLKFVRA PMVVQQPPVQ361PQVQQQQTAV QTAQAAQMVA PGVQVSQSSL PMLSSPSPGQ QVQTPQSMPP PPQPSPQPGQ421PSSQPNSNVS SGPAPSPSSF LPSPSPQPSQ SPVTARTPQN FSVPSPGPLN TPVNPSSVMS481PAGSSQAEEQ QYLDKLKQLS KYIEPLRRMI NKIDKNEDRK KDLSKMKSLL DILTDPSKRC541PLKTLQKCEI ALEKLKNDMA VPTPPPPPVP PTKQQYLCQP LLDAVLANIR SPVFNHSLYR601TFVPAMTAIH GPPITAPVVC TRKRRLEDDE RQSIPSVLQG EVARLDPKFL VNLDPSHCSN661NGTVHLICKL DDKDLPSVPP LELSVPADYP AQSPLWIDRQ WQYDANPFLQ SVHRCMTSRL721LQLPDKHSVT ALLNTWAQSV HQACLSAATRTRPLEQKLISEEDLAANDILDYKDDDDKV
748 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.