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MDGA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MDGA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MDGA1 antibodies →
$725.00each
Catalog Number: LS-G105741-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MDGA1 (NM_153487), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAMDC3 | MDGA1 | GPIM | GPI and MAM protein
Origin Species
Human
SwissProt
Q8NFP4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVTCLLLLA LIPFHCRGQG VYAPAQAQIV HAGQACVVKE DNISERVYTI REGDTLMLQC
61LVTGHPRPQV RWTKTAGSAS DKFQETSVFN ETLRIERIAR TQGGRYYCKA ENGVGVPAIK
121SIRVDVQYLD EPMLTVHQTV SDVRGNFYQE KTVFLRCTVN SNPPARFIWK RGSDTLSHSQ
181DNGVDIYEPL YTQGETKVLK LKNLRPQDYA SYTCQVSVRN VCGIPDKAIT FRLTNTTAPP
241ALKLSVNETL VVNPGENVTV QCLLTGGDPL PQLQWSHGPG PLPLGALAQG GTLSIPSVQA
301RDSGYYNCTA TNNVGNPAKK TVNLLVRSMK NATFQITPDV IKESENIQLG QDLKLSCHVD
361AVPQEKVTYQ WFKNGKPARM SKRLLVTRND PELPAVTSSL ELIDLHFSDY GTYLCMASFP
421GAPVPDLSVE VNISSETVPP TISVPKGRAV VTVREGSPAE LQCEVRGKPR PPVLWSRVDK
481EAALLPSGLP LEETPDGKLR LERVSRDMSG TYRCQTARYN GFNVRPREAQ VQLNVQFPPE
541VEPSSQDVRQ ALGRPVLLRC SLLRGSPQRI ASAVWRFKGQ LLPPPPVVPA AAEAPDHAEL
601RLDAVTRDSS GSYECSVSND VGSAACLFQV SAKAYSPEFY FDTPNPTRSH KLSKNYSYVL
661QWTQREPDAV DPVLNYRLSI RQLNQHNAVV KAIPVRRVEK GQLLEYILTD LRVPHSYEVR
721LTPYTTFGAG DMASRIIHYT EPINSPNLSD NTCHFEDEKI CGYTQDLTDN FDWTRQNALT
781QNPKRSPNTG PPTDISGTPE GYYMFIETSR PRELGDRARL VSPLYNASAK FYCVSFFYHM
841YGKHIGSLNL LVRSRNKGAL DTHAWSLSGN KGNVWQQAHV PISPSGPFQI IFEGVRGPGY
901LGDIAIDDVT LKKGECPRKQ TDPNKVVVMP GSGAPCQSSP QLWGPMAIFL LALQR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

955 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.