Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MCRS1 / MSP58 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121826-100
Catalog Number: LS-G121826
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MCRS1 (NM_001012300), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 58 kDa microspherule protein | INO80 complex subunit Q | INO80 complex subunit J | INO80Q | MCRS1 | MCRS2 | Microspherule protein 1 | MSP58 | ICP22BP | p78
- Origin Species
- Human
- NCBI GenBank
- NM_006337
- SwissProt
- Q96EZ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTRGTGGTAQ RGRSGPDSQG LLDSSLMASG TASRSEDEES LAGQKRASSQ ALGTIPKRRS61SSRFIKRKKF DDELVESSLA KSSTRAKGAS GVEPGRCSGS EPSSSEKKKV SKAPSTPVPP121SPAPAPGLTK RVKKSKQPLQ VTKDLGRWKP ADDLLLINAV LQTNDLTSVH LGVKFSCRFT181LREVQERWYA LLYDPVISKL ACQAMRQLHP EAIAAIQSKA LFSKAEEQLL SKVGSTSQPT241LETFQDLLHR HPDAFYLART AKALQAHWQL MKQYYLLEDQ TVQPLPKGDQ VLNFSDAEDL301IDDSKLKDMR DEVLEHELMV ADRRQKREIR QLEQELHKWQ VLVDSITGMS SPDFDNQTLA361VLRGRMVRYL MRSREITLGR ATKDNQIDVD LSLEGPAWKI SRKQGVIKLK NNGDFFIANE421GRRPIYIDGR PVLCGSKWRL SNNSVVEIAS LRFVFLINQD LIALIRAEAA KITPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
475 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.