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MCPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MCPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109902-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human BRIT1(MCPH1) (NM_024596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRIT1 | MCT | Microcephalin | MCPH1 | Microcephalin 1
Origin Species
Human
NCBI GenBank
NM_024596
SwissProt
Q8NEM0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPILKDVV AYVEVWSSNG TENYSKTFTT QLVDMGAKVS KTFNKQVTHV IFKDGYQSTW
61DKAQKRGVKL VSVLWVEKCR TAGAHIDESL FPAANMNEHL SSLIKKKRKC MQPKDFNFKT
121PENDKRFQKK FEKMAKELQR QKTNLDDDVP ILLFESNGSL IYTPTIEINS SHHSAMEKRL
181QEMKEKRENL SPTSSQMIQQ SHDNPSNSLC EAPLNISRDT LCSDEYFAGG LHSSFDDLCG
241NSGCGNQERK LEGSINDIKS DVCISSLVLK ANNIHSSPSF THLDKSSPQK FLSNLSKEEI
301NLQRNIAGKV VTPHQKQAAG MSQETFEEKY RLSPTLSSTK GHLLIHSRPR SSSVKRKRVS
361HGSHSPPKEK CKRKRSTRRS IMPRLQLCRS EGRLQHVAGP ALEALSCGES SYDDYFSPDN
421LKERYSENLP PESQLPSSPA QLSCRSLSKK ERTSIFEMSD FSCVGKKTRT VDITNFTAKT
481ISSPRKTGNG EGRATSSCVT SAPEEALRCC RQAGKEDACP EGNGFSYTIE DPALPKGHDD
541DLTPLEGSLE EMKEAVGLKS TQNKGTTSKI SNSSEGEAQS EHEPCFIVDC NMETSTEEKE
601NLPGGYSGSV KNRPTRHDVL DDSCDGFKDL IKPHEELKKS GRGKKPTRTL VMTSMPSEKQ
661NVVIQVVDKL KGFSIAPDVC ETTTHVLSGK PLRTLNVLLG IARGCWVLSY DWVLWSLELG
721HWISEEPFEL SHHFPAAPLC RSECHLSAGP YRGTLFADQP AMFVSPASSP PVAKLCELVH
781LCGGRVSQVP RQASIVIGPY SGKKKATVKY LSEKWVLDSI TQHKVCAPEN YLLSQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

835 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.