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MCM10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MCM10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MCM10 antibodies →
$504.00each
Catalog Number: LS-G101577-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MCM10 (NM_018518), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CNA43 | HsMCM10 | MCM10 | Homolog of yeast MCM10 | PRO2249 | DNA43 | Protein MCM10 homolog
Origin Species
Human
NCBI GenBank
NM_018518
SwissProt
Q7L590

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG DGESYTEEAD
61DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV LPAPAPRREK TNEELQEELR
121NLQEQMKALQ EQLKVTTIKQ TASPARLQKS PVEKSPRPPL KERRVQRIQE STCFSAELDV
181PALPRTKRVA RTPKASPPDP KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE
241TTQPICVEAF SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI
301LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV VGILNANPMK
361PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC TQTVNLRDCE YCQYHVQAQY
421KKLSAKRADL QSTFSGGRIP KKFARRGTSL KERLCQDGFY YGGVSSASYA ASIAAAVAPK
481KKIQTTLSNL VVKGTNLIIQ ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA
541TASGIMGSPK PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS
601RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK LSALAEAKKL
661AAITKLRARG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK NTMFSSQAED ELEPARKKRR
721EQLAYLESEE FQKILKAKSK HTGILKEAEA EMQERYFEPL VKKEQMEEKM RNIREVKCRV
781VTCKTCAYTH FKLLETCVSE QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK
841WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

875 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.