Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MCF2L / DBS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124252-100
Catalog Number: LS-G124252
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ARHGEF14(MCF2L) (NM_001112732), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARHGEF14 | DBS | KIAA0362 | MCF2L | OST | DBLs big sister
- Origin Species
- Human
- NCBI GenBank
- NM_024979
- SwissProt
- O15068
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 131.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARVESPVPA ARASLTGSCV LGQAMPLRGG AGPSPASHGP THGPSDPRTC LPGRGAGGMR61PHGRGALGCC GLCSFYTCHG AAGDEIMHQD IVPLCAADIQ DQLKKRFAYL SGGRGQDGSP121VITFPDYPAF SEIPDKEFQN VMTYLTSIPS LQDAGIGFIL VIDRRRDKWT SVKASVLRIA181ASFPANLQLV LVLRPTGFFQ RTLSDIAFKF NRDDFKMKVP VIMLSSVPDL HGYIDKSQLT241EDLGGTLDYC HSRWLCQRTA IESFALMVKQ TAQMLQSFGT ELAETELPND VQSTSSVLCA301HTEKKDKAKE DLRLALKEGH SVLESLRELQ AEGSEPSVNQ DQLDNQATVQ RLLAQLNETE361AAFDEFWAKH QQKLEQCLQL RHFEQGFREV KAILDAASQK IATFTDIGNS LAHVEHLLRD421LASFEEKSGV AVERARALSL DGEQLIGNKH YAVDSIRPKC QELRHLCDQF SAEIARRRGL481LSKSLELHRR LETSMKWCDE GIYLLASQPV DKCQSQDGAE AALQEIEKFL ETGAENKIQE541LNAIYKEYES ILNQDLMEHV RKVFQKQASM EEVFHRRQAS LKKLAARQTR PVQPVAPRPE601ALAKSPCPSP GIRRGSENSS SEGGALRRGP YRRAKSEMSE SRQGRGSAGE EEESLAILRR661HVMSELLDTE RAYVEELLCV LEGYAAEMDN PLMAHLLSTG LHNKKDVLFG NMEEIYHFHN721RIFLRELENY TDCPELVGRC FLERMEDFQI YEKYCQNKPR SESLWRQCSD CPFFQECQRK781LDHKLSLDSY LLKPVQRITK YQLLLKEMLK YSRNCEGAED LQEALSSILG ILKAVNDSMH841LIAITGYDGN LGDLGKLLMQ GSFSVWTDHK RGHTKVKELA RFKPMQRHLF LHEKAVLFCK901KREENGEGYE KAPSYSYKQS LNMAAVGITE NVKGDAKKFE IWYNAREEVY IVQAPTPEIK961AAWVNEIRKV LTSQLQACRE ASQHRALEQS QSLPLPAPTS TSPSRGNSRN IKKLEERKTD1021PLSLEGYVSS APLTKPPEKG KGWSKTSHSL EAPEDDGGWS SAEEQINSSD AEEDGGLGPK1081KLVPGKYTVV ADHEKGGPDA LRVRSGDVVE LVQEGDEGLW YVRDPTTGKE GWVPASSLSV1141RLGPSGSAQC LSSSESSPGS AVLSNSSSCS EGGQAPFSDL QGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1182 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.