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MBIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MBIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MBIP antibodies →
$504.00each
Catalog Number: LS-G112559-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K12 binding inhibitory protein 1(MBIP) (NM_016586), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MBIP | MUK-binding inhibitory protein
Origin Species
Human
NCBI GenBank
NM_016586
SwissProt
Q9NS73

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
39.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAATEHNRP SSGDRNLERR CSPNLSREVL YEIFRSLHTL VGQLDLRDDV VKITIDWNKL
61QSLSAFQPAL LFSALEQHIL YLQPFLAKLQ SPIKEENTTA VEEIGRTEMG NKNEVNDKFS
121IGDLQEEEKH KESDLRDVKK TQIHFDPEVV QIKAGKAEID RRISAFIERK QAEINENNVR
181EFCNVIDCNQ ENSCARTDAI FTPYPGFKSH VKVSRVVYTY GPQTRPEGIP GSGHKPNSML
241RDCGNQAVEE RLQNIEAHLR LQTGGPVPRD IYQRIKKLED KILELEGISP EYFQSVSFSG
301KRRKVQPPQQ NYSLAELDEK ISALKQALLR KSREAESMAT HHLPTRTRPL EQKLISEEDL
361AANDILDYKD DDDKV

375 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.