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MB21D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MB21D2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MB21D2 antibodies →
$504.00each
Catalog Number: LS-G104637-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf59(MB21D2) (NM_178496), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C3orf59 | MB21D2 | Mab-21 domain containing 2 | Protein MB21D2
Origin Species
Human
SwissProt
Q8IYB1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAPTANKAA SLGCNNKPAF PELDFRSGAR VEELNKLIQE FTKHDQREYD DQRALEIHTA
61KDFIFSMLGM VQKLDQKLPV ANEYLLLSGG VREGVVDLDL DELNVYARGT DYDMDFTLLV
121PALKLHDRNQ PVTLDMRHSA LCHSWLSLRL FDEGTISKWK DCCTIVDHIN GATNYFFSPT
181KVADWFYDSI SIVLSEIQKK PQRGMPKVEK VEKNGTIISI ILGVGSSRML YDIVPVVSFK
241GWPAVAQSWL MENHFWDGKI TEEEVISGFY LVPACSYKGK KDNEWRLSFA RSEVQLKKCI
301SSSLMQAYQA CKAIIIKLLS RPKAISPYHL RSMMLWACDR LPANYLAQED YAAHFLLGLI
361DDLQHCLVNK MCPNYFIPQC NMLEHLSEET VMLHARKLSS VRSDPAEHLR TAIEHVKAAN
421RLTLELQRRG STTSIPSPQS DGGDPNQPDD RLAKKLQQLV TENPGKSISV FINPDDVTRP
481HFRIDDKFF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

489 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.