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MAZ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAZ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAZ antibodies →
$725.00each
Catalog Number: LS-G119575-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAZ (NM_001042539), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAZ | Pur-1 | SAF-2 | SAF-3 | MAZI | ZF87 | Zif87 | ZNF801 | Transcription factor Zif87 | Zinc finger protein 801 | PUR1 | SAF-1
Origin Species
Human
NCBI GenBank
NM_002383
SwissProt
P56270

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFPVFPCTLL APPFPVLGLD SRGVGGLMNS FPPPQGHAQN PLQVGAELQS RFFASQGCAQ
61SPFQAAPAPP PTPQAPAAEP LQVDLLPVLA AAQESAAAAA AAAAAAAAVA AAPPAPAAAS
121TVDTAALKQP PAPPPPPPPV SAPAAEAAPP ASAATIAAAA ATAVVAPTST VAVAPVASAL
181EKKTKSKGPY ICALCAKEFK NGYNLRRHEA IHTGAKAGRV PSGAMKMPTM VPLSLLSVPQ
241LSGAGGGGGE AGAGGGAAAV AAGGVVTTTA SGKRIRKNHA CEMCGKAFRD VYHLNRHKLS
301HSDEKPYQCP VCQQRFKRKD RMSYHVRSHD GAVHKPYNCS HCGKSFSRPD HLNSHVRQVH
361STERPFKCEK CEAAFATKDR LRAHTVRHEE KVPCHVCGKM LSSAYISDHM KVHSQGPHHV
421CELCNKGFTT AAYLRIHAVK DHGLQAPRAD RILCKLCSVH CKTPAQLAGH MQTHLGGAAP
481PVPGDAPQPQ PTC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.