Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MATN4 / Matrilin 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117061-100
Catalog Number: LS-G117061
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Matrilin 4(MATN4) (NM_003833), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HE6WCR54 | MATN4 | Matrilin-4 | Matrilin 4
- Origin Species
- Human
- NCBI GenBank
- NM_003833
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.02 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRGLLCWPVL LLLLQPWETQ LQLTGPRCHT GPLDLVFVID SSRSVRPFEF ETMRQFLMGL61LRGLNVGPNA TRVGVIQYSS QVQSVFPLRA FSRREDMERA IRDLVPLAQG TMTGLAIQYA121MNVAFSVAEG ARPPEERVPR VAVIVTDGRP QDRVAEVAAQ ARASGIEIYA VGVQRADVGS181LRAMASPPLD EHVFLVESFD LIQEFGLQFQ SRLCAIDLCA EGTHGCEHHC VNSPGSYFCH241CQVGFVLQQD QRSCRAIDYC SFGNHSCQHE CVSTPGGPRC HCREGHDLQP DGRSCQVRDL301CNGVDHGCEF QCVSEGLSYR CLCPEGRQLQ ADGKSCNRCR EGHVDLVLLV DGSKSVRPQN361FELVKRFVNQ IVDFLDVSPE GTRVGLVQFS SRVRTEFPLG RYGTAAEVKQ AVLAVEYMER421GTMTGLALRH MVEHSFSEAQ GARPRALNVP RVGLVFTDGR SQDDISVWAA RAKEEGIVMY481AVGVGKAVEA ELREIASEPA ELHVSYAPDF GTMTHLLENL RGSICPEEGI SAGTELRSPC541ECESLVEFQG HTLGALESLT LNLAQLTARL EDLENQLANQ KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
581 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.