Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MATN2 / Matrilin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109477-100
Catalog Number: LS-G109477
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Matrilin 2(MATN2) (NM_030583), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Matrilin-2 | MATN2 | Matrilin 2
- Origin Species
- Human
- NCBI GenBank
- NM_002380
- SwissProt
- O00339
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 102.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKMLAGCFL LILGQIVLLP AEARERSRGR SISRGRHART HPQTALLESS CENKRADLVF61IIDSSRSVNT HDYAKVKEFI VDILQFLDIG PDVTRVGLLQ YGSTVKNEFS LKTFKRKSEV121ERAVKRMRHL STGTMTGLAI QYALNIAFSE AEGARPLREN VPRVIMIVTD GRPQDSVAEV181AAKARDTGIL IFAIGVGQVD FNTLKSIGSE PHEDHVFLVA NFSQIETLTS VFQKKLCTAH241MCSTLEHNCA HFCINIPGSY VCRCKQGYIL NSDQTTCRIQ DLCAMEDHNC EQLCVNVPGS301FVCQCYSGYA LAEDGKRCVA VDYCASENHG CEHECVNADG SYLCQCHEGF ALNPDEKTCT361KIDYCASSNH GCQHECVNTD DSYSCHCLKG FTLNPDKKTC RRINYCALNK PGCEHECVNM421EESYYCRCHR GYTLDPNGKT CSRVDHCAQQ DHGCEQLCLN TEDSFVCQCS EGFLINEDLK481TCSRVDYCLL SDHGCEYSCV NMDRSFACQC PEGHVLRSDG KTCAKLDSCA LGDHGCEHSC541VSSEDSFVCQ CFEGYILRED GKTCRRKDVC QAIDHGCEHI CVNSDDSYTC ECLEGFRLAE601DGKRCRRKDV CKSTHHGCEH ICVNNGNSYI CKCSEGFVLA EDGRRCKKCT EGPIDLVFVI661DGSKSLGEEN FEVVKQFVTG IIDSLTISPK AARVGLLQYS TQVHTEFTLR NFNSAKDMKK721AVAHMKYMGK GSMTGLALKH MFERSFTQGE GARPLSTRVP RAAIVFTDGR AQDDVSEWAS781KAKANGITMY AVGVGKAIEE ELQEIASEPT NKHLFYAEDF STMDEISEKL KKGICEALED841SDGRQDSPAG ELPKTVQQPT VQHRYLFEED NLLRSTQKLS HSTKPSGSPL EEKHDQCKCE901NLIMFQNLAN EEVRKLTQRL EEMTQRMEAL ENRLRYRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
937 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.