Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAST205 / MAST2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101292-100
Catalog Number: LS-G101292
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAST205(MAST2) (NM_015112), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0807 | MAST2 | MTSSK | RP4-533D7.1 | MAST205
- Origin Species
- Human
- NCBI GenBank
- NM_015112
- SwissProt
- Q6P0Q8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 196.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKRSRCRDRP QPPPPDRRED GVQRAAELSQ SLPPRRRAPP GRQRLEERTG PAGPEGKEQD61VVTGVSPLLF RKLSNPDIFS STGKVKLQRQ LSQDDCKLWR GNLASSLSGK QLLPLSSSVH121SSVGQVTWQS SGEASNLVRM RNQSLGQSAP SLTAGLKELS LPRRGSFCRT SNRKSLIVTS181STSPTLPRPH SPLHGHTGNS PLDSPRNFSP NAPAHFSFVP ARRTDGRRWS LASLPSSGYG241TNTPSSTVSS SCSSQEKLHQ LPFQPTADEL HFLTKHFSTE SVPDEEGRQS PAMRPRSRSL301SPGRSPVSFD SEIIMMNHVY KERFPKATAQ MEERLAEFIS SNTPDSVLPL ADGALSFIHH361QVIEMARDCL DKSRSGLITS QYFYELQDNL EKLLQDAHER SESSEVAFVM QLVKKLMIII421ARPARLLECL EFDPEEFYHL LEAAEGHAKE GQGIKCDIPR YIVSQLGLTR DPLEEMAQLS481SCDSPDTPET DDSIEGHGAS LPSKKTPSEE DFETIKLISN GAYGAVFLVR HKSTRQRFAM541KKINKQNLIL RNQIQQAFVE RDILTFAENP FVVSMFCSFD TKRHLCMVME YVEGGDCATL601LKNIGALPVD MVRLYFAETV LALEYLHNYG IVHRDLKPDN LLITSMGHIK LTDFGLSKIG661LMSLTTNLYE GHIEKDAREF LDKQVCGTPE YIAPEVILRQ GYGKPVDWWA MGIILYEFLV721GCVPFFGDTP EELFGQVISD EIVWPEGDEA LPPDAQDLTS KLLHQNPLER LGTGSAYEVK781QHPFFTGLDW TGLLRQKAEF IPQLESEDDT SYFDTRSERY HHMDSEDEEE VSEDGCLEIR841QFSSCSPRFN KVYSSMERLS LLEERRTPPP TKRSLSEEKE DHSDGLAGLK GRDRSWVIGS901PEILRKRLSV SESSHTESDS SPPMTVRRRC SGLLDAPRFP EGPEEASSTL RRQPQEGIWV961LTPPSGEGVS GPVTEHSGEQ RPKLDEEAVG RSSGSSPAME TRGRGTSQLA EGATAKAISD1021LAVRRARHRL LSGDSTEKRT ARPVNKVIKS ASATALSLLI PSEHHTCSPL ASPMSPHSQS1081SNPSSRDSSP SRDFLPALGS MRPPIIIHRA GKKYGFTLRA IRVYMGDSDV YTVHHMVWHV1141EDGGPASEAG LRQGDLITHV NGEPVHGLVH TEVVELILKS GNKVAISTTP LENTSIKVGP1201ARKGSYKAKM ARRSKRSRGK DGQERKRSSL FRKITKQASL LHTSRSLSSL NRSLSSGESG1261PGSPTHSHSL SPRSPTQGYR VTPDAVHSVG GNSSQSSSPS SSVPSSPAGS GHTRPSSLHG1321LAPKLQRQYR SPRRKSAGSI PLSPLAHTPS PPPPTASPQR SPSPLSGHVA QAFPTKLHLS1381PPLGRQLSRP KSAEPPRSPL LKRVQSAEKL AAALAASEKK LATSRKHSLD LPHSELKKEL1441PPREVSPLEV VGARSVLSGK GALPGKGVLQ PAPSRALGTL RQDRAERRES LQKQEAIREV1501DSSEDDTEEG PENSQGAQEL SLAPHPEVSQ SVAPKGAGES GEEDPFPSRD PRSLGPMVPS1561LLTGITLGPP RMESPSGPHR RLGSPQAIEE AASSSSAGPN LGQSGATDPI PPEGCWKAQH1621LHTQALTALS PSTSGLTPTS SCSPPSSTSG KLSMWSWKSL IEGPDRASPS RKATMAGGLA1681NLQDLENTTP AQPKNLSPRE QGKTQPPSAP RLAHPSYEDP SQGWLWESEC AQAVKEDPAL1741SITQVPDASG DRRQDVPCRG CPLTQKSEPS LRRGQEPGGH QKHRDLALVP DELLKQTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1797 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.