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MAST1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAST1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAST1 antibodies →
$725.00each
Catalog Number: LS-G113539-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAST1 (NM_014975), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SAST | KIAA0973 | MAST1 | SAST170
Origin Species
Human
NCBI GenBank
AB023190
SwissProt
Q9Y2H9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
170.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH SPLPGHLGSS
61PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG TNTPSSTVSS SCSSQERLHQ
121LPYQPTVDEL HFLSKHFGST ESITDEDGGR RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH
181VYKERFPKAT AQMEEKLRDF TRAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI
241TTVYFYELQE NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY
301HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVH LEEQDSGGSN TPEQDDLSEG
361RSSKAKKPPG ENDFDTIKLI SNGAYGAVYL VRHRDTRQRF AMKKINKQNL ILRNQIQQAF
421VERDILTFAE NPFVVGMFCS FETRRHLCMV MEYVEGGDCA TLLKNIGALP VEMARMYFAE
481TVLALEYLHN YGIVHRDLKP DNLLITSMGH IKLTDFGLSK MGLMSLTTNL YEGHIEKDAR
541EFLDKQVCGT PEYIAPEVIL RQGYGKPVDW WAMGIILYEF LVGCVPFFGD TPEELFGQVI
601SDDILWPEGD EALPTEAQLL ISSLLQTNPL VRLGAGGAFE VKQHSFFRDL DWTGLLRQKA
661EFIPHLESED DTSYFDTRSD RYHHVNSYDE DDTTEEEPVE IRQFSSCSPR FSKVYSSMEQ
721LSQHEPKTPV AAAGSSKREP STKGPEEKVA GKREGLGGLT LREKTWRGGS PEIKRFSASE
781ASFLEGEASP PLGARRRFSA LLEPSRFSAP QEDEDEARLR RPPRPSSDPA GSLDARAPKE
841ETQGEGTSSA GDSEATDRPR PGDLCPPSKD GDASGPRATN DLVLRRARHQ QMSGDVAVEK
901RPSRTGGKVI KSASATALSV MIPAVDPHGS SPLASPMSPR SLSSNPSSRD SSPSRDYSPA
961VSGLRSPITI QRSGKKYGFT LRAIRVYMGD TDVYSVHHIV WHVEEGGPAQ EAGLCAGDLI
1021THVNGEPVHG MVHPEVVELI LKSGNKVAVT TTPFENTSIR IGPARRSSYK AKMARRNKRP
1081SAKEGQESKK RSSLFRKITK QSNLLHTSRS LSSLNRSLSS SDSLPGSPTH GLPARSPTHS
1141YRSTPDSAYL GASSQSSSPA SSTPNSPASS ASHHIRPSTL HGLSPKLHRQ YRSARCKSAG
1201NIPLSPLAHT PSPTQASPPP LPGHTVGSSH TTQSFPAKLH SSPPVVRPRP KSAEPPRSPL
1261LKRVQSAEKL GASLSADKKG ALRKHSLEVG HPDFRKDFHG ELALHSLAES DGETPPVEGL
1321GAPRQVAVRR LGRQESPLSL GADPLLPEGA SRPPVSSKEK ESPGGAEACT PPRATTPGGR
1381TLERDVGCTR HQSVQTEDGT GGMARAVAKA ALSPVQEHET GRRSSSGEAG TPLVPIVVEP
1441ARPGAKAVVP QPLGADSKGL QEPAPLAPSV PEAPRGRERW VLEVVEERTT LSGPRSKPAS
1501PKLSPEPQTP SLAPAKCSAP SSAVTPVPPA SLLGSGTKPQ VGLTSRCPAE AVPPAGLTKK
1561GVSSPAPPGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1570 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.