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MARS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MARS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107368-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MARS2 (NM_138395), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MARS2 | Methionyl-tRNA synthetase 2 | MtMetRS | Methionine-tRNA ligase | Methionine--tRNA ligase 2 | MetRS
Origin Species
Human
NCBI GenBank
NM_138395
SwissProt
Q96GW9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLRTSVLRLL GRTGASRLSL LEDFGPRYYS SGSLSAGDDA CDVRAYFTTP IFYVNAAPHI
61GHLYSALLAD ALCRHRRLRG PSTAATRFST GTDEHGLKIQ QAAATAGLAP TELCDRVSEQ
121FQQLFQEAGI SCTDFIRTTE ARHRVAVQHF WGVLKSRGLL YKGVYEGWYC ASDECFLPEA
181KVTQQPGPSG DSFPVSLESG HPVSWTKEEN YIFRLSQFRK PLQRWLRGNP QAITPEPFHH
241VVLQWLDEEL PDLSVSRRSS HLHWGIPVPG DDSQTIYVWL DALVNYLTVI GYPNAEFKSW
301WPATSHIIGK DILKFHAIYW PAFLLGAGMS PPQRICVHSH WTVCGQKMSK SLGNVVDPRT
361CLNRYTVDGF RYFLLRQGVP NWDCDYYDEK VVKLLNSELA DALGGLLNRC TAKRINPSET
421YPAFCTTCFP SEPGLVGPSV RAQAEDYALV SAVATLPKQV ADHYDNFRIY KALEAVSSCV
481RQTNGFVQRH APWKLNWESP VDAPWLGTVL HVALECLRVF GTLLQPVTPS LADKLLSRLG
541VSASERSLGE LYFLPRFYGH PCPFEGRRLG PETGLLFPRL DQSRTWLVKA HRT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

593 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.