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MAPKBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAPKBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAPKBP1 antibodies →
$725.00each
Catalog Number: LS-G113554-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAPKBP1 (NM_014994), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
JNKBP1 | MAPKBP1 | JNK-binding protein 1 | JNKBP-1 | KIAA0596
Origin Species
Human
NCBI GenBank
AB011168
SwissProt
O60336

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
163 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVEGSTITS RIKNLLRSPS IKLRRSKAGN RREDLSSKVT LEKVLGITVS GGRGLACDPR
61SGLVAYPAGC VVVLFNPRKH KQHHILNSSR KTITALAFSP DGKYLVTGES GHMPAVRVWD
121VAEHSQVAEL QEHKYGVACV AFSPSAKYIV SVGYQHDMIV NVWAWKKNIV VASNKVSSRV
181TAVSFSEDCS YFVTAGNRHI KFWYLDDSKT SKVNATVPLL GRSGLLGELR NNLFTDVACG
241RGKKADSTFC ITSSGLLCEF SDRRLLDKWV ELRTTVAHCI SVSQDYIFCG CADGTVRLFN
301PSNLHFLSTL PRPHALGTDI ASVTEASRLF SGVANARYPD TIALTFDPTN QWLSCVYNDH
361SIYVWDVRDP KKVGKVYSAL YHSSCVWSVE VYPEVKDSNQ ACLPPSSFIT CSSDNTIRLW
421NTESSGVHGS TLHRNILSSD LIKIIYVDGN TQALLDTELP GGDKADASLL DPRVGIRSVC
481VSPNGQHLAS GDRMGTLRVH ELQSLSEMLK VEAHDSEILC LEYSKPDTGL KLLASASRDR
541LIHVLDAGRE YSLQQTLDEH SSSITAVKFA ASDGQVRMIS CGADKSIYFR TAQKSGDGVQ
601FTRTHHVVRK TTLYDMDVEP SWKYTAIGCQ DRNIRIFNIS SGKQKKLFKG SQGEDGTLIK
661VQTDPSGIYI ATSCSDKNLS IFDFSSGECV ATMFGHSEIV TGMKFSNDCK HLISVSGDSC
721IFVWRLSSEM TISMRQRLAE LRQRQRGGKQ QGPSSPQRAS GPNRHQAPSM LSPGPALSSD
781SDKEGEDEGT EEELPALPVL AKSTKKALAS VPSPALPRSL SHWEMSRAQE SVGFLDPAPA
841ANPGPRRRGR WVQPGVELSV RSMLDLRQLE TLAPSLQDPS QDSLAIIPSG PRKHGQEALE
901TSLTSQNEKP PRPQASQPCS YPHIIRLLSQ EEGVFAQDLE PAPIEDGIVY PEPSDNPTMD
961TSEFQVQAPA RGTLGRVYPG SRSSEKHSPD SACSVDYSSS CLSSPEHPTE DSESTEPLSV
1021DGISSDLEEP AEGDEEEEEE EGGMGPYGLQ EGSPQTPDQE QFLKQHFETL ASGAAPGAPV
1081QVPERSESRS ISSRFLLQVQ TRPLREPSPS SSSLALMSRP AQVPQASGEQ PRGNGANPPG
1141APPEVEPSSG NPSPQQAASV LLPRCRLNPD SSWAPKRVAT ASPFSGLQKA QSVHSLVPQE
1201RHEASLQAPS PGALLSREIE AQDGLGSLPP ADGPPSRPHS YQNPTTSSMA KISRSISVGE
1261NLGLVAEPQA HAPIRVSPLS KLALPSRAHL VLDIPKPLPD RPTLAAFSPV TKGRAPGEAE
1321KPGFPVGLGK AHSTTERWAC LGEGTTPKPR TECQAHPGPS SPCAQQLPVS SLFQGPENLQ
1381PPPPEKTPNP MECTKPGAAL SQDSEPAVSL EQCEQLVAEL RGSVRQAVRL YHSVAGCRMP
1441SAEQSRIAQL LRDTFSSVRQ ELEAVAGAVL SSPGSSPGAV GAEQTQALLE QYSELLLRAV
1501ERRMERKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.