Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MAPK8IP2 / JIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112690-100
Catalog Number: LS-G112690
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JIP2(MAPK8IP2) (NM_016431), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Homologous to mouse JIP-1 | Islet-brain-2 | JNK-interacting protein 2 | IB-2 | IB2 | Islet-brain 2 | PRKM8 interacting protein-like | MAPK8IP2 | JIP-2 | JIP2 | PRKM8IPL
- Origin Species
- Human
- NCBI GenBank
- NM_012324
- SwissProt
- Q13387
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPDFPSPST WAPGLLLPSG PALLSPSVLQ DSLSLGRSEQ PHPICSFQDD FQEFEMIDDN61EEEDDEDEEE EEEEEEGDGE GQEGGDPGSE APAPGPLIPS PSVEEPHKHR PTTLRLTTLG121AQDSLNNNGG FDLVRPASWQ ETALCSPAPE ALRELPGPLP ATDTGPGGAQ SPVRPGCDCE181GNRPAEPPAP GGTSPSSDPG IEADLRSRSS GGRGGRRSSQ ELSSPGSDSE DAGGARLGRM241ISSISETELE LSSDGGSSSS GRSSHLTNSI EEASSPASEP EPPREPPRRP AFLPVGPDDT301NSEYESGSES EPDLSEDADS PWLLSNLVSR MISEGSSPIR CPGQCLSPAP RPPGEPVSPA361GGAAQDSQDP EAAAGPGGVE LVDMETLCAP PPPAPAAPRP GPAQPGPCLF LSNPTRDTIT421PLWAAPGRAA RPGRACSAAC SEEEDEEDDE EEEDAEDSAG SPGGRGTGPS APRDASLVYD481AVKYTLVVDE HTQLELVSLR RCAGLGHDSE EDSGGEASEE EAGAALLGGG QVSGDTSPDS541PDLTFSKKFL NVFVNSTSRS SSTESFGLFS CLVNGEEREQ THRAVFRFIP RHPDELELDV601DDPVLVEAEE DDFWFRGFNM RTGERGVFPA FYAHAVPGPA KDLLGSKRSP CWVERFDVQF661LGSVEVPCHQ GNGILCAAMQ KIATARKLTV HLRPPASCDL EISLRGVKLS LSGGGPEFQR721CSHFFQMKNI SFCGCHPRNS CYFGFITKHP LLSRFACHVF VSQESMRPVA QSVGRAFLEY781YQEHLAYACP TEDIYLESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
797 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.