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MAP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MAP9 antibodies →
$725.00each
Catalog Number: LS-G120628-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP9 (NM_001039580), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Aster-associated protein | ASAP | MAP9
Origin Species
Human
NCBI GenBank
AK024812
SwissProt
Q49MG5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDEVFSTTL AYTKSPKVTK RTTFQDELIR AITARSARQR SSEYSDDFDS DEIVSLGDFS
61DTSADENSVN KKMNDFHISD DEEKNPSKLL FLKTNKSNGN ITKDEPVCAI KNEEEMAPDG
121CEDIVVKSFS ESQNKDEEFE KDKIKMKPKP RILSIKSTSS AENNSLDTDD HFKPSPRPRS
181MLKKKSHMEE KDGLEDKETA LSEELELHSA PSSLPTPNGI QLEAEKKAFS ENLDPEDSCL
241TSLASSSLKQ ILGDSFSPGS EGNASGKDPN EEITENHNSL KSDENKENSF SADHVTTAVE
301KSKESQVTAD DLEEEKAKAE LIMDDDRTVD PLLSKSQSIL ISTSATASSK KTIEDRNIKN
361KKSTNNRASS ASARLMTSEF LKKSSSKRRT PSTTTSSHYL GTLKVLDQKP SQKQSIEPDR
421ADNIRAAVYQ EWLEKKNVYL HEMHRIKRIE SENLRIQNEQ KKAAKREEAL ASFEAWKAMK
481EKEAKKIAAK KRLEEKNKKK TEEENAARKG EALQAFEKWK EKKMEYLKEK NRKEREYERA
541KKQKEEETVA EKKKDNLTAV EKWNEKKEAF FKQKEKEKIN EKRKEELKRA EKKDKDKQAI
601NEYEKWLENK EKQERIERKQ KKRHSFLESE ALPPWSPPSR TVFAKVF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

647 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.