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MAP7D3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP7D3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109904-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP7D3 (NM_024597), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_024597

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMADGAAAGA GGSPSLRELR ARMVAAANEI AKERRKQDVV NRVATHSSNI RSTFKPVIDG
61SMLKNDIKQR LARERREEKR RQQDANKETQ LLEKERKTKL QYEKQMEERQ RKLKERKEKE
121EQRRIAAEEK RHQKDEAQKE KFTAILYRTL ERRRLADDYQ QKRWSWGGSA MANSESKTAN
181KRSASTEKLE QGTSALIRQM PLSSAGLQNS VAKRKTDKER SSSLNRRDSN LHSSTDKEQA
241ERKPRVTGVT NYVMQYVTVP LRKCTSDELR AVMFPMSTMK IPPQTKVEES PLEKVETPPK
301ASVDAPPQVN VEVFCNTSME ASPKAGVGMA PEVSTDSFPV VSVDVSPVVS TYDSEMSMDA
361SPELSIEALP KVDLETVPKV SIVASPEASL EAPPEVSLEA LPEVSVEAAP EGSLEAPPKG
421SAEVAPKESV KGSPKESMEA SPEAMVKASP KTSLEASMEA SPKAKARDAP KKSEMDKQAL
481IPIAKKRLSS YTECYKWSSS PENACGLPSP ISTNRQIQKN CPPSPLPLIS KQSPQTSFPY
541KIMPIQHTLS VQSASSTVKK KKETVSKTTN RCEALSQRHM IYEESGNKST AGIMNAEAAT
601KILTELRRLA REQREKEEEE RQREEMQQRV IKKSKDMAKE AVGGQAEDHL KLKDGQQQNE
661TKKKKGWLDQ EDQEAPLQKG DAKIKAQEEA DKRKKEHERI MLQNLQERLE RKKRIEEIMK
721RTRKTDVNAS KVTETSSHDI YEEAEADNEE SDKDSLNEMF PSAILNGTGS PTKFKMPFNN
781AKKMTHKLVF LEDGTSQVRK EPKTYFNGDL KNFRQKSMKD TSIQEVVSRP SSKRMTSHTT
841KTRKADETNT TSRSSAQTKS EGFHDILPKS SDTFRQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

876 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.