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MAP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108471-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP6 (NM_033063), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAP-6 | MTAP6 | KIAA1878 | Stable tubule-only polypeptide | STOP | N-STOP | MAP6
Origin Species
Human
NCBI GenBank
N90813
SwissProt
Q96JE9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAWPCITRAC CIARFWNQLD KADIAVPLVF TKYSEATEHP GAPPQPPPPQ QQAQPALAPP
61SARAVAIETQ PAQGELDAVA RATGPAPGPT GEREPAAGPG RSGPGPGLGS GSTSGPADSV
121MRQDYRAWKV QRPEPSCRPR SEYQPSDAPF ERETQYQKDF RAWPLPRRGD HPWIPKPVQI
181SAASQASAPI LGAPKRRPQS QERWPVQAAA EAREQEAAPG GAGGLAAGKA SGADERDTRR
241KAGPAWMVRR AEGLGHEQTP LPAAQAQVQA TGPEAGRGRA AADALNRQIR EEVASAVSSS
301YRNEFRAWTD IKPVKPIKAK PQYKPPDDKM VHETSYSAQF KGEASKPTTA DNKVIDRRRI
361RSLYSEPFKE PPKVEKPSVQ SSKPKKTSAS HKPTRKAKDK QAVSGQAAKK KSAEGPSTTK
421PDDKEQSKEM NNKLAEAKES LAQPVSDSSK TQGPVATEPD KDQGSVVPGL LKGQGPMVQE
481PLKKQGSVVP GPPKDLGPMI PLPVKDQDHT VPEPLKNESP VISAPVKDQG PSVPVPPKNQ
541SPMVPAKVKD QGSVVPESLK DQGPRIPEPV KNQAPMVPAP VKDEGPMVSA SVKDQGPMVS
601APVKDQGPIV PAPVKGEGPI VPAPVKDEGP MVSAPIKDQD PMVPEHPKDE SAMATAPIKN
661QGSMVSEPVK NQGLVVSGPV KDQDVVVPEH AKVHDSAVVA PVKNQGPVVP ESVKNQDPIL
721PVLVKDQGPT VLQPPKNQGR IVPEPLKNQV PIVPVPLKDQ DPLVPVPAKD QGPAVPEPLK
781TQGPRDPQLP TVSPLPRVMI PTAPHTEYIE SSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

813 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.