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MAP4K5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MAP4K5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103560-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP4K5 (NM_198794), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Germinal center kinase-related | KHS | KHS-PEN | MEK kinase kinase 5 | MAPKKKK5 | MEKKK 5 | KHS1 | MAP4K5
Origin Species
Human
NCBI GenBank
NM_006575
SwissProt
Q9Y4K4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAPLRPAAD ILRRNPQQDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI IKLEPGDDFS
61LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG SLQDIYHVTG PLSELQIAYV
121CRETLQGLAY LHTKGKMHRD IKGANILLTD HGDVKLADFG VAAKITATIA KRKSFIGTPY
181WMAPEVAAVE KNGGYNQLCD IWAVGITAIE LGELQPPMFD LHPMRALFLM SKSNFQPPKL
241KDKTKWSSTF HNFVKIALTK NPKKRPTAER LLTHTFVAQP GLSRALAVEL LDKVNNPDNH
301AHYTEADDDD FEPHAIIRHT IRSTNRNARA ERTASEINFD KLQFEPPLRK ETEARDEMGL
361SSDPNFMLQW NPFVDGANTG KSTSKRAIPP PLPPKPRISS YPEDNFPDEE KASTIKHCPD
421SESRAPQILR RQSSPSCGPV AETSSIGNGD GISKLMSENT EGSAQAPQLP RKKDKRDFPK
481PAINGLPPTP KVLMGACFSK VFDGCPLKIN CATSWIHPDT KDQYIIFGTE DGIYTLNLNE
541LHEATMEQLF PRKCTWLYVI NNTLMSLSEG KTFQLYSHNL IALFEHAKKP GLAAHIQTHR
601FPDRILPRKF ALTTKIPDTK GCHKCCIVRN PYTGHKYLCG ALQSGIVLLQ WYEPMQKFML
661IKHFDFPLPS PLNVFEMLVI PEQEYPMVCV AISKGTESNQ VVQFETINLN SASSWFTEIG
721AGSQQLDSIH VTQLERDTVL VCLDKFVKIV NLQGKLKSSK KLASELSFDF RIESVVCLQD
781SVLAFWKHGM QGKSFKSDEV TQEISDETRV FRLLGSDRVV VLESRPTENP TAHSNLYILA
841GHENSY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

846 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.