LSBio
MAP3K12 Protein (Over-Expression Lysate Myc-DDK (Flag))

MAP3K12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115406-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP3K12 (NM_006301), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dual leucine zipper kinase DLK | Dlk | Dual leucine zipper kinase | Leucine zipper protein kinase | Leucine-zipper protein kinase | MAPK-upstream kinase | MEKK12 | Mixed lineage kinase | Protein kinase MUK | MAP3K12 | MUK | ZPK | ZPKP1
Origin Species
Human
NCBI GenBank
NM_006301
SwissProt
Q12852

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MACLHETRTP SPSFGGFVST LSEASMRKLD PDTSDCTPEK DLTPTHVLQL HEQDAGGPGG
61AAGSPESRAS RVRADEVRLQ CQSGSGFLEG LFGCLRPVWT MIGKAYSTEH KQQQEDLWEV
121PFEEILDLQW VGSGAQGAVF LGRFHGEEVA VKKVRDLKET DIKHLRKLKH PNIITFKGVC
181TQAPCYCILM EFCAQGQLYE VLRAGRPVTP SLLVDWSMGI AGGMNYLHLH KIIHRDLKSP
241NMLITYDDVV KISDFGTSKE LSDKSTKMSF AGTVAWMAPE VIRNEPVSEK VDIWSFGVVL
301WELLTGEIPY KDVDSSAIIW GVGSNSLHLP VPSSCPDGFK ILLRQCWNSK PRNRPSFRQI
361LLHLDIASAD VLSTPQETYF KSQAEWREEV KLHFEKIKSE GTCLHRLEEE LVMRRREELR
421HALDIREHYE RKLERANNLY MELNALMLQL ELKERELLRR EQALERRCPG LLKPHPSRGL
481LHGNTMEKLI KKRNVPQKLS PHSKRPDILK TESLLPKLDA ALSGVGLPGC PKGPPSPGRS
541RRGKTRHRKA SAKGSCGDLP GLRTAVPPHE PGGPGSPGGL GGGPSAWEAC PPALRGLHHD
601LLLRKMSSSS PDLLSAALGS RGRGATGGAG DPGSPPPARG DTPPSEGSAP GSTSPDSPGG
661AKGEPPPPVG PGEGVGLLGT GREGTSGRGG SRAGSQHLTP AALLYRAAVT RSQKRGISSE
721EEEGEVDSEV ELTSSQRWPQ SLNMRQSLST FSSENPSDGE EGTASEPSPS GTPEVGSTNT
781DERPDERSDD MCSQGSEIPL DPPPSEVIPG PEPSSLPIPH QELLRERGPP NSEDSDCDST
841ELDNSNSVDA LRPPASLPP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

859 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.